Magnetic alginate microparticles for purification of α-amylases

被引：51

作者：

Safaríková, M

Roy, I

Gupta, MN

Safarík, I

机构：

[1] Inst Landscape Ecol, Lab Biochem & Microbiol, Ceske Budejovice 37005, Czech Republic

[2] Indian Inst Technol, Dept Chem, New Delhi 110016, India

[3] Univ S Bohemia, Dept Gen Biol, Ceske Budejovice 37005, Czech Republic

来源：

JOURNAL OF BIOTECHNOLOGY | 2003年 / 105卷 / 03期

关键词：

alginate; ferrofluid; magnetic fluid; magnetic microparticles; magnetic nanoparticles; Amylases;

D O I：

10.1016/j.jbiotec.2003.07.002

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Spherical magnetic alginate microparticles (25-60 mum in diameter) were prepared using the microemulsion system, with water-saturated 1-pentanol as the organic phase. The limited solubility of 1-pentanol in water enabled simple removal of the organic solvent from the prepared beads with water solution. The prepared alginate microparticles were used as magnetic affinity adsorbents for specific purification of alpha-amylases. Enzyme activity was eluted by 1.0 M maltose. alpha-Amylases from Bacillus amyloliquefaciens and porcine pancreatic acetone powder were purified 9- and 12-fold with 88 and 96% activity recovery, respectively. (C) 2003 Elsevier B.V. All rights reserved.

引用

页码：255 / 260

页数：6

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