Characterization of a spontaneous nonmagnetic mutant of Magnetospirillum gryphiswaldense reveals a large deletion comprising a putative magnetosome island

被引:151
作者
Schübbe, S
Kube, M
Scheffel, A
Wawer, C
Heyen, U
Meyerdierks, A
Madkour, MH
Mayer, F
Reinhardt, R
Schüler, D
机构
[1] Max Planck Inst Marine Mikrobiol, D-28359 Bremen, Germany
[2] Max Planck Inst Mol Genet, D-14195 Berlin, Germany
[3] Univ Gottingen, Inst Mikrobiol & Genet, D-37077 Gottingen, Germany
关键词
D O I
10.1128/JB.185.19.5779-5790.2003
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Frequent spontaneous loss of the magnetic phenotype was observed in stationary-phase cultures of the magnetotactic bacterium Magnetospirillum gryphiswaldense MSR-1. A nonmagnetic mutant, designated strain MSR-1B, was isolated and characterized. The mutant lacked any structures resembling magnetosome crystals as well as internal membrane vesicles. The growth of strain MSR-1B was impaired under all growth conditions tested, and the uptake and accumulation of iron were drastically reduced under iron-replete conditions. A large chromosomal deletion of approximately 80 kb was identified in strain MSR-1B, which comprised both the entire mamAB and mamDC clusters as well as further putative operons encoding a number of magnetosome-associated proteins. A bacterial artificial chromosome clone partially covering the deleted region was isolated from the genomic library of wild-type M. gryphiswaldense. Sequence analysis of this fragment revealed that all previously identified mam genes were closely linked with genes encoding other magnetosome-associated proteins within less than 35 kb. In addition, this region was remarkably rich in insertion elements and harbored a considerable number of unknown gene families which appeared to be specific for magnetotactic bacteria. Overall, these findings suggest the existence of a putative large magnetosome island in M. gryphiswaldense and other magnetotactic bacteria.
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页码:5779 / 5790
页数:12
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