Functional characterization of human mesenchymal stem cell-derived adipocytes

被引：100

作者：

Rydén, M ^{[1
]}

Dicker, A

Götherström, C

Åström, G

Tammik, C

Arner, P

Le Blanc, K

机构：

[1] Huddinge Univ Hosp, Karolinska Inst, Ctr Metab & Endocrinol, Dept Med, S-14186 Stockholm, Sweden

[2] Huddinge Univ Hosp, Karolinska Inst, Div Immunol, Dept Lab Med, S-14186 Stockholm, Sweden

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2003年 / 311卷 / 02期

关键词：

stem cell; differentiation; adipocyte; lipolysis; antilipolysis; alpha 2A adrenoceptor; HSL; PDE;

D O I：

10.1016/j.bbrc.2003.10.010

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The function of adipocytes derived from human mesenchymal stem cells (hMSC) was investigated for the first time in hMSC from fetal liver (FL) and adult bone marrow (BM) and compared with preadipocytes from human subcutaneous adipose tissue differentiated according to adipocyte-specific protocols. FL- and BM-derived adipocytes displayed both morphological and functional characteristics of mature adipocytes including specific intracellular signaling pathways for tumor necrosis factor-alpha, catecholamine-regulated lipolysis as well as secretion of adiponectin and leptin. Similar to differentiated preadipocytes, hMSC adipocytes displayed lipolytic effects mediated by beta-adrenoceptors and antilipolytic effects mediated by the alpha2A-adrenoceptor (alpha2A-AR) and expressed proteins with a pivotal role in human lipolysis, including beta2-AR, alpha2A-AR, and hormone-sensitive lipase. We conclude that hMSC-derived adipocytes are morphologically and functionally similar to preadipocytes and display an intact lipolytic signaling pathway and endocrine function. These systems could be of great value in adipocyte research as a renewable source of adipocytes. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：391 / 397

页数：7

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