N-Nitrogodiethylamine mutagenicity at low concentrations

被引：38

作者：

Aiub, CAF

Pinto, LFR

Felzenszwalb, I

机构：

[1] Univ Estado Rio de Janeiro, Inst Biol Roberto Alcantara Gomes, Dept Biofis & Biometria, BR-20551030 Rio De Janeiro, Brazil

[2] Univ Estado Rio de Janeiro, Inst Biol Roberto Alcantara Gomes, Dept Bioquim, BR-20551030 Rio De Janeiro, Brazil

来源：

TOXICOLOGY LETTERS | 2003年 / 145卷 / 01期

关键词：

NDEA; SOS chromotest; Salmonella/microsome assay; metabolic activation;

D O I：

10.1016/S0378-4274(03)00263-7

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 [卫生毒理学];

摘要：

N-Nitrosodiethylamine (NDEA) requires metabolic activation by cytochrome P450 enzymes, leading to electrophile species that react in DNA. Although, carcinogenicity is not an end point in genotoxicity assays, NDEA has been considered a weak carcinogen. In this study, we carried out an analysis of the mutagenicity at low concentrations of NDEA. Using SOS chromotest in the presence of metabolic activation, we detected positive mutagenicity response for NDEA doses between 0.75 and 36.46 mug/ml. In Ames test, using more sensitive strains in the presence of S9 metabolic activation mixture (S9 mix), positive results were also detected for NDEA doses between 1.01 x 10(-3) and 50.64 x 10(-3) mug per plate. Our results indicate that NDEA mutagenicity can be detected at low concentrations when more sensitive conditions are used. (C) 2003 Elsevier Ireland Ltd. All rights reserved.

引用

页码：36 / 45

页数：10

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