Xylanases from Cellulomonas flavigena: purification and characterization

被引：7

作者：

Horcasitas, CM ^{[1
]}

Lopez, JO ^{[1
]}

Plaza, IM ^{[1
]}

机构：

[1] Inst Politecn Nacl, Ctr Invest & Estudios Avanzados, Dept Biotecnol & Bioingn, Mexico City 07360, DF, Mexico

来源：

BIOTECHNOLOGY TECHNIQUES | 1998年 / 12卷 / 09期

关键词：

D O I：

10.1023/A:1008852618130

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Three xylanases (Xyl1, Xyl2 and Xyl3) were purified and characterized from the culture supernatant of Cellulomonas flavigena grown on sugar cane bagasse. The enzymes were purified by affinity chromatography and gel filtration and had masses of 63 kDa, 17 kDa and 35 kDa, respectively as measured by SDS-PAGE. All enzymes were active against 4-O-methyl-D-glucuronoxylan and xylan but had no cellulase activity with CM-cellulose, an important characteristics in biobleaching processes.

引用

页码：663 / 666

页数：4

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