Catecholamine regulation of local lactate production in vivo in skeletal muscle and adipose tissue:: Role of β-adrenoreceptor subtypes

被引:35
作者
Qvisth, Veronica [1 ]
Hagstrom-Toft, Eva [1 ]
Enoksson, Staffan [2 ]
Bolinder, Jan [1 ]
机构
[1] Karolinska Univ, Huddinge Hosp, Karolinska Inst, Dept Med, SE-14186 Stockholm, Sweden
[2] Karolinska Univ, Hosp Sonia, Karolinska Inst, Dept Vasc Surg, SE-17176 Stockholm, Sweden
关键词
D O I
10.1210/jc.2007-1313
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Context: The regulation of lactate production in skeletal muscle (SM) and adipose tissue (AT) is not fully elucidated. Objective: Our objective was to investigate the catecholamine-mediated regulation of lactate production and blood flow in SM and AT in healthy, normal-weight subjects by using microdialysis. Methods: First, lactate levels in SM and AT were measured during an iv norepinephrine infusion (n = 11). Local blood flow was determined with the Xe-133-clearance technique. Second, muscle lactate was measured during hypoglycemia and endogenous epinephrine stimulation (n = 12). Third, SM was perfused with selective beta(1-3)-adrenoreceptor agonists in situ(n = 8). Local blood flow was measured with the ethanol perfusion technique. Results: In response to iv norepinephrine, the fractional release of lactate (difference between tissue and arterial lactate) increased by 40% in SM (P = 0.001), whereas remaining unchanged in AT. Blood flow decreased by 40% in SM (P < 0.005) and increased by 50% in AT (P < 0.05). In response to hypoglycemia, epinephrine increased 10-fold, and the fractional release of lactate in SM doubled (P < 0.0001). The blood flow remained unchanged. The beta(2)-agonist, terbutaline, caused a marked concentration-dependent increase of muscle lactate and blood flow (P < 0.0001). The beta(1)-agonist, dobutamine, induced a discrete increase of muscle lactate (P < 0.0001), and the blood flow remained unchanged. The beta(3)-agonist, CPG 12177, did not affect muscle lactate or blood flow. Conclusions: Catecholamines stimulate lactate production in SM, but not in AT. In SM, the beta(2)-adrenoreceptor is the most important beta-adrenergic receptor subtype in the regulation of lactate production.
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页码:240 / 246
页数:7
相关论文
共 45 条
[1]   Evidence for a secondary state of the human β3-adrenoceptor [J].
Baker, JG .
MOLECULAR PHARMACOLOGY, 2005, 68 (06) :1645-1655
[2]  
Bergrneyer HV, 1974, METHOD ENZYMAT AN, V3, P1468
[3]  
Bernst E, 1974, METHOD ENZYMAT AN, P1499
[4]   Lactate shuttles in nature [J].
Brooks, GA .
BIOCHEMICAL SOCIETY TRANSACTIONS, 2002, 30 :258-264
[5]   HEMODYNAMIC-EFFECTS OF PHYSIOLOGICAL CONCENTRATIONS OF CIRCULATING NORADRENALINE IN MAN [J].
CHANG, PC ;
KRIEK, E ;
VANDERKROGT, JA ;
BLAUW, GJ ;
VANBRUMMELEN, P .
CLINICAL SCIENCE, 1988, 75 (05) :469-475
[6]   VASCULAR AND ENDOCRINE CONTROL OF MUSCLE METABOLISM [J].
CLARK, MG ;
COLQUHOUN, EQ ;
RATTIGAN, S ;
DORA, KA ;
ELDERSHAW, TPD ;
HALL, JL ;
YE, JM .
AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM, 1995, 268 (05) :E797-E812
[7]   MECHANISM OF INCREASED GLUCONEOGENESIS IN NONINSULIN-DEPENDENT DIABETES-MELLITUS - ROLE OF ALTERATIONS IN SYSTEMIC, HEPATIC, AND MUSCLE LACTATE AND ALANINE METABOLISM [J].
CONSOLI, A ;
NURJHAN, N ;
REILLY, JJ ;
BIER, DM ;
GERICH, JE .
JOURNAL OF CLINICAL INVESTIGATION, 1990, 86 (06) :2038-2045
[8]   Hypoglycaemia: The limiting factor in the glycaemic management of Type I and Type II Diabetes [J].
Cryer, PE .
DIABETOLOGIA, 2002, 45 (07) :937-948
[9]   Beta3-adrenoceptor agonists as anti-diabetic and anti-obesity drugs in humans [J].
de Souza, CJ ;
Burkey, BF .
CURRENT PHARMACEUTICAL DESIGN, 2001, 7 (14) :1433-1449
[10]   EPINEPHRINE-INDUCED INSULIN RESISTANCE IN MAN [J].
DEIBERT, DC ;
DEFRONZO, RA .
JOURNAL OF CLINICAL INVESTIGATION, 1980, 65 (03) :717-721