Simultaneous measurement of foliar glutathione, γ-glutamylcysteine, and amino acids by high-performance liquid chromatography:: Comparison with two other assay methods for glutathione

被引：145

作者：

Noctor, G ^{[1
]}

Foyer, CH ^{[1
]}

机构：

[1] Inst Grassland & Environm Res, Dept Environm Biol, Plas Gogerddan SY23 3EB, Ceredigion, Wales

来源：

ANALYTICAL BIOCHEMISTRY | 1998年 / 264卷 / 01期

关键词：

D O I：

10.1006/abio.1998.2794

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The recent production of transformed plants with enhanced capacity for glutathione synthesis has highlighted the interactions between foliar glutathione and turnover of free amino acid pools. The development of a convenient method for simultaneous measurement of glutathione, gamma-glutamylcysteine, and 16 amino acids is reported. This method utilizes derivatization of compounds with o-phthalaldehyde in the presence of a-mercaptoethanol followed by separation using reversed-phase high-performance liquid chromatography, fluted compounds are detected fluorimetrically. The method was tested using untransformed poplars and poplars in which foliar thiol contents have been enhanced by overexpression of gamma-glutamylcysteine synthetase. Foliar contents of glutathione determined by this method were comparable to those measured in common extracts by two other techniques. (C) 1998 Academic Press.

引用

页码：98 / 110

页数：13

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