Two-Photon Fluorescence Lysosomal Bioimaging with a Micelle-Encapsulated Fluorescent Probe

被引：27

作者：

Andrade, Carolina D. ^{[1
]}

Yanez, Ciceron O. ^{[1
]}

Qaddoura, Maher A. ^{[1
]}

Wang, Xuhua ^{[1
]}

Arnett, Curtesa L. ^{[1
]}

Coombs, Sabrina A. ^{[1
]}

Yu, Jin ^{[1
]}

Bassiouni, Rania ^{[1
]}

Bondar, Mykhailo V. ^{[3
]}

Belfield, Kevin D. ^{[1
,2
]}

机构：

[1] Univ Cent Florida, Dept Chem, Orlando, FL 32816 USA

[2] Univ Cent Florida, CREOL, Coll Opt & Photon, Orlando, FL 32816 USA

[3] Natl Acad Sci Ukraine, Inst Phys, Dept Photoact, Kiev, Ukraine

来源：

JOURNAL OF FLUORESCENCE | 2011年 / 21卷 / 03期

基金：

美国国家卫生研究院; 美国国家科学基金会;

关键词：

Fluorescent dyes; Pluronic; Multiphoton absorption; Bioimaging; Fluorescence microscopy; Lysosomes; STIMULATED-EMISSION-DEPLETION; OPTICAL-DATA STORAGE; FLUORENE DERIVATIVES; CROSS-SECTIONS; BECLIN; MICROSCOPY; AUTOPHAGY; RESOLUTION; TRANSPORT; NM;

D O I：

10.1007/s10895-010-0801-3

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We report two-photon fluorescence microscopy (2PFM) imaging and in vitro cell viability of a new, efficient, lysosome-selective system based on a two-photon absorbing (2PA) fluorescent probe (I) encapsulated in Pluronic (R) F-127 micelles. Preparation of dye I was accomplished via microwave-assisted synthesis, resulting in improved yields and reduced reaction times. Photophysical characterization revealed notable 2PA efficiency of this probe.

引用

页码：1223 / 1230

页数：8

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