General transcriptional repression by polyglutamine disease proteins is not directly linked to the presence of inclusion bodies

被引：13

作者：

Hoshino, M

Tagawa, K

Okuda, T

Okazawa, H

机构：

[1] Tokyo Metropolitan Inst Neurosci, Dept Mol Therapeut, Fuchu, Tokyo 1838526, Japan

[2] Tokyo Med & Dent Univ, Inst Med Res, Dept Neuropathol, Bunkyo Ku, Tokyo 1138510, Japan

[3] PRESTO, Japan Sci & Technol Corp JST, Kawagoe, Saitama 3320012, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2004年 / 313卷 / 01期

关键词：

D O I：

10.1016/j.bbrc.2003.11.086

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

By using direct immunocytochemistry of BrU incorporated to RNA in the nuclei, we evaluated the effect of mutant huntingtin and ataxin-1 on general transcription in primary cortical and cerebellar neurons. Our quantitative analyses clearly showed that these mutant polyglutamine disease proteins repress general transcription. In addition, we found that general transcription level was almost similar in inclusion body-positive and -negative neurons. The result suggests that presence of inclusion body is not essential for repressing general transcription in contrast to its reported role for suppressing specific gene transcription in the polyglutamine disease pathology. (C) 2003 Elsevier Inc. All rights reserved.

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页码：110 / 116

页数：7

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