Fluorescent multiplex analysis of nine STR loci: Spanish population data

被引：17

作者：

Entrala, C

Lorente, M

Lorente, JA

Alvarez, JC

Moretti, T

Budowle, B

Villanueva, E

机构：

[1] Univ Granada, Dept Legal Med, Fac Med, Lab Genet Identificat, E-18012 Granada, Spain

[2] Fed Bur Invest Acad, Ctr Forens Sci Res & Training, Lab Div, Forens Sci Res Unit, Quantico, VA 22135 USA

来源：

FORENSIC SCIENCE INTERNATIONAL | 1998年 / 98卷 / 03期

关键词：

Spanish population; STR; PCR; forensic genetics; fluorescent;

D O I：

10.1016/S0379-0738(98)00165-0

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

A total of 171 Caucasians living in Andalucia (southern Spain) have been typed for nine short tandem repeat (STR) loci by multiplex PCR amplification using a commercially available kit (Profiler Plus; Perkin-Elmer, Norwalk, CT, USA) and semi-automatic electrophoresis (ABI Prism 377 DNA Sequencer, Applied Biosystems, Foster City, CA, USA). The kit enables typing of the STR loci D3S1358, VWA, FGA, D5S818, D13S317, D7S820, D8S1179, D21S11, and D18S51. All loci, except D7S820, meet Hardy-Weinberg equilibrium. Because of the large number of loci that can be analyzed, the power of discrimination (PD) is greater than 0.99999, and the probability of exclusion (PE) reaches 0.99991 in our population sample. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：179 / 183

页数：5

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