Identification of hyperinvasive Campylobacter jejuni strains isolated from poultry and human clinical sources

被引:51
作者
Fearnley, Catherine [1 ]
Manning, Georgina [2 ]
Bagnall, Mary [1 ]
Javed, Muhammad Afzal [2 ]
Wassenaar, Trudy M. [3 ]
Newell, Diane G. [1 ]
机构
[1] Vet Labs Agcy, Weybridge KT15 3NB, Surrey, England
[2] Nottingham Trent Univ, Sch Sci & Technol, Nottingham NG11 8NS, England
[3] Mol Microbiol & Genom Consultants, Zotzenheim, Germany
关键词
D O I
10.1099/jmm.0.47803-0
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Campylobacter jejuni causes gastroenteritis with a variety of symptoms in humans. In the absence of a suitable animal model, in vitro models have been used to study virulence traits such as invasion and toxin production. In this study, 113 C. jejuni isolates from poultry and poultry-related (n=74) environments as well as isolates from human cases (n=39) of campylobacteriosis and bacteraemia were tested for invasiveness using INT 407 cells. The method was sufficiently reproducible to observe a spectrum of invasiveness amongst strains. As a result, strains were classified as low, high and hyper-invasive. The majority of strains (poultry and human) were low invaders (82% and 88%, respectively), High invasion was found for 5% of human strains and 11% of poultry-related isolates. However, only 1% of poultry strains were classified as hyperinvasive compared to 13% of human isolates (P=0.0182). Of those isolates derived from the blood of bacteraemic patients, 20% were hyperinvasive, though this correlation was not statistically significant. An attempt was made to correlate invasiveness with the presence of seven genes previously reported to be associated with virulence. Most of these genes did not correlate with invasiveness, but gene cj0486 was weakly over-represented, and a negative correlation was observed for the gene ciaB. This trend was stronger when the two genes were analysed together, thus ciaB(-) cj0486(+) was over-represented in high and hyperinvasive strains, with low invaders more commonly found to lack these genes (P=0.0064).
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页码:570 / 580
页数:11
相关论文
共 65 条
[1]   Cytolethal distending toxin (CDT)-negative Campylobacter jejuni strains and anti-CDT neutralizing antibodies are induced during human infection but not during colonization in chickens [J].
AbuOun, M ;
Manning, G ;
Cawthraw, SA ;
Ridley, A ;
Ahmed, IH ;
Wassenaar, TM ;
Newell, DG .
INFECTION AND IMMUNITY, 2005, 73 (05) :3053-3062
[2]  
Adak GK, 2005, EMERG INFECT DIS, V11, P365
[3]   NEUROTENSIN AND NEUROMEDIN-N STIMULATE MUCIN OUTPUT FROM HUMAN GOBLET CELLS (CL.16E) VIA NEUROTENSIN RECEPTORS [J].
AUGERON, C ;
VOISIN, T ;
MAORET, JJ ;
BERTHON, B ;
LABURTHE, M ;
LABOISSE, CL .
AMERICAN JOURNAL OF PHYSIOLOGY, 1992, 262 (03) :G470-G476
[4]   DNA sequence and mutational analyses of the pVir plasmid of Campylobacter jejuni 81-176 [J].
Bacon, DJ ;
Alm, RA ;
Hu, L ;
Hickey, TE ;
Ewing, CP ;
Batchelor, RA ;
Trust, TJ ;
Guerry, P .
INFECTION AND IMMUNITY, 2002, 70 (11) :6242-6250
[5]   Involvement of a plasmid in virulence of Campylobacter jejuni 81-176 [J].
Bacon, DJ ;
Alm, RA ;
Burr, DH ;
Hu, L ;
Kopecko, DJ ;
Ewing, CP ;
Trust, TJ ;
Guerry, P .
INFECTION AND IMMUNITY, 2000, 68 (08) :4384-4390
[6]   PCR detection of seven virulence and toxin genes of Campylobacter jejuni and Campylobacter coli isolates from Danish pigs and cattle and cytolethal distending toxin production of the isolates [J].
Bang, DD ;
Nielsen, EM ;
Scheutz, F ;
Pedersen, K ;
Handberg, K ;
Madsen, M .
JOURNAL OF APPLIED MICROBIOLOGY, 2003, 94 (06) :1003-1014
[7]   Prevalence of cytolethal distending toxin (cdt) genes and CDT production in Campylobacter spp. isolated from Danish broilers [J].
Bang, DD ;
Scheutz, F ;
Ahrens, P ;
Pedersen, K ;
Blom, J ;
Madsen, M .
JOURNAL OF MEDICAL MICROBIOLOGY, 2001, 50 (12) :1087-1094
[8]   Uptake pathways of clinical and healthy animal isolates of Campylobacter jejuni into INT-407 cells [J].
Biswas, D ;
Itoh, K ;
Sasakawa, C .
FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY, 2000, 29 (03) :203-211
[9]   Invasive ability of an Escherichia coli strain isolated from the ileal mucosa of a patient with Crohn's disease [J].
Boudeau, J ;
Glasser, AL ;
Masseret, E ;
Joly, B ;
Darfeuille-Michaud, A .
INFECTION AND IMMUNITY, 1999, 67 (09) :4499-4509
[10]   Transcellular translocation of Campylobacter jejuni across human polarised epithelial monolayers [J].
Brás, AM ;
Ketley, JM .
FEMS MICROBIOLOGY LETTERS, 1999, 179 (02) :209-215