The biocompatibility evaluation of epoxy resin-based root canal sealers in vitro

被引：57

作者：

Huang, TH

Yang, JJ

Li, H

Kao, CT

机构：

[1] Chung Shan Med & Dent Coll, Inst Stomatol, Taichung 402, Taiwan

[2] Chung Shan Med & Dent Coll, Dept Dent, Sect 1, Taichung 402, Taiwan

[3] Chung Shan Med & Dent Coll, Inst Toxicol, Sect 1, Taichung 402, Taiwan

来源：

BIOMATERIALS | 2002年 / 23卷 / 01期

关键词：

biocompatibility of epoxy resin sealer; MTT assay; alkaline single cell gel electrophoresis;

D O I：

10.1016/S0142-9612(01)00081-3

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 [生物医学工程];

摘要：

The cytotoxic and mutagenic effects of epoxy resin-based root canal sealer AH26 and AH-Plus were determined in vitro. Root canal sealers were eluted for 24h in dimethyl sulfoxide (DMSO) and diluted in culture medium. Cytotoxic effects were assessed using the MTT (tetrazolium dye, 3-(4.5-dimethylthiazol-2-yl)-2.5-diphenyltetrazolium bromide, C(18)H(16)N5SBr] assay for mitochondrial enzyme activity and also the cell viability. Genotoxicity assays were assessed using the alkaline single cell gel electrophoresis assay (comet assay) for DNA damage measurement. Result indicated that both the AH26 and AH-Plus sealers exhibited a dose-dependent increase in astrocyte toxic effects. Additionally, dose-dependent astrocyte DNA damage was also noted for both sealers. Therefore, these epoxy resin-based sealers, AH26 and AH-Plus demonstrated both cytotoxicity and genotoxicity in vitro. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

页码：77 / 83

页数：7

共 31 条

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METABOLISM OF ARACHIDONIC-ACID TO EPOXYEICOSATRIENOIC ACIDS, HYDROXYEICOSATETRAENOIC ACIDS, AND PROSTAGLANDINS IN CULTURED RAT HIPPOCAMPAL ASTROCYTES [J].