Polyethylene glycol improves the purification of recombinant human tumor necrosis factor during ion exchange chromatography

被引：15

作者：

Feng, XL

Gu, ZY

Jin, YT

Su, ZG ^{[2
]}

机构：

[1] Dalian Univ Technol, Sch Chem Engn, Biochem Engn Lab, Dalian 116012, Peoples R China

[2] Chinese Acad Sci, Inst Chem Met, State Key Lab Biochem Engn, Beijing 100080, Peoples R China

来源：

BIOTECHNOLOGY TECHNIQUES | 1998年 / 12卷 / 04期

基金：

中国国家自然科学基金;

关键词：

D O I：

10.1023/A:1008850214356

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Poly(ethylene glycol) (PEG) - either PEG 200, 600 or 4000 - when added to the buffer and feed solution gave nearly one-fold increase in the purification and about 100% recovery of recombinant human tumor necrosis factor-alpha during anion-exchange chromatography. The optimum concentration was 1% with the best result being achieved with PEG 200.

引用

页码：289 / 293

页数：5

共 12 条

[1] AGGARWAL BB, 1985, J BIOL CHEM, V260, P2345
[2] MECHANISM OF POLY(ETHYLENE GLYCOL) INTERACTION WITH PROTEINS
ARAKAWA, T
TIMASHEFF, SN
[J]. BIOCHEMISTRY, 1985, 24 (24) : 6756 - 6762
[3] POLYETHYLENE-GLYCOL ENHANCED PROTEIN REFOLDING
CLELAND, JL
BUILDER, SE
SWARTZ, JR
WINKLER, M
CHANG, JY
WANG, DIC
[J]. BIO-TECHNOLOGY, 1992, 10 (09): : 1013 - 1019
[4] STRUCTURE OF HUMAN-TUMOR NECROSIS FACTOR-ALPHA DERIVED FROM RECOMBINANT-DNA
DAVIS, JM
NARACHI, MA
ALTON, NK
ARAKAWA, T
[J]. BIOCHEMISTRY, 1987, 26 (05) : 1322 - 1326
[5] FOURNIERE L, 1995, BIOCHIM BIOPHYS ACTA, V1248, P186
[6] Method for obtaining unique selectivities in ion-exchange chromatography by addition of organic polymers to the mobile phase
Gagnon, P
Godfrey, B
Ladd, D
[J]. JOURNAL OF CHROMATOGRAPHY A, 1996, 743 (01) : 51 - 55
[7] STRUCTURE OF TUMOR NECROSIS FACTOR
JONES, EY
STUART, DI
WALKER, NPC
[J]. NATURE, 1989, 338 (6212) : 225 - 228
[8] THERMAL-STABILITY OF PROTEINS IN THE PRESENCE OF POLY(ETHYLENE GLYCOLS)
LEE, LLY
LEE, JC
[J]. BIOCHEMISTRY, 1987, 26 (24) : 7813 - 7819
[9] LIGNY CLD, 1984, J CHROMATOGR, V294, P223
[10] PURIFICATION AND CHARACTERIZATION OF A HUMAN-TUMOR NECROSIS FACTOR FROM THE LUKII CELL-LINE
RUBIN, BY
ANDERSON, SL
SULLIVAN, SA
WILLIAMSON, BD
CARSWELL, EA
OLD, LJ
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1985, 82 (19) : 6637 - 6641

← 1 2 →