Charge displacements in interfacial layers containing reaction centers

被引:9
作者
Brzezinski, P
Messinger, A
Blatt, Y
Gopher, A
Kleinfeld, D
机构
[1] Univ Gothenburg, Dept Biochem & Biophys, SE-40530 Gothenburg, Sweden
[2] Chalmers Univ Technol, SE-40530 Gothenburg, Sweden
[3] Salk Inst Biol Studies, La Jolla, CA 92037 USA
[4] Bio Dar Ltd, IL-81103 Yavne, Israel
[5] Chaim Sheba Med Ctr, Inst Pharmacol & Toxicol, IL-52621 Tel Hashomer, Israel
[6] Univ Calif San Diego, Dept Phys, La Jolla, CA 92093 USA
关键词
bacterial photosynthesis; electrogenic event; charge transfer; electron transfer; monolayer;
D O I
10.1007/s002329900435
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Reaction centers from the photosynthetic bacterium Rhodobacter sphaeroides were oriented in phospholipid interfacial layers adsorbed to a Teflon film separating two electrolyte-filled compartments of a Teflon cell. Light-induced voltage changes were measured as a function of time across electrodes immersed in the cell compartments. The experimental system is characterized both experimentally and theoretically to relate the measured signals to the light-induced displacement currents in the reaction centers. Mathematical relations between the measured signals and the distances and geometries of the charge-transfer reactions are derived. At pH 8.0 the reaction centers were found to be oriented with similar to 60% of the population oriented with the donor facing the aqueous phase. The density of the reaction centers in the layer was similar to 10(11) cm(-2), which is close to that found in the native system. Reconstitution of the secondary quinone, Q(B), in 90% of the RCs was achieved with an similar to 100-fold excess of ubiquinone in the vesicle preparation.
引用
收藏
页码:213 / 225
页数:13
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