Anti-Apoptotic and Pro-Survival Effect of Alpinate Oxyphyllae Fructus (AOF) in a D-Galactose-Induced Aging Heart

被引：56

作者：

Chang, Yung-Ming ^{[1
,2
,3
]}

Chang, Hen-Hong ^{[4
,5
,6
]}

Kuo, Wei-Wen ^{[7
]}

Lin, Hung-Jen ^{[5
,6
]}

Yeh, Yu-Lan ^{[8
,9
]}

Viswanadha, Vijaya Padma ^{[10
]}

Tsai, Chin-Chuan ^{[1
,2
]}

Chen, Ray-Jade ^{[11
]}

Chang, Hsin-Nung ^{[12
]}

Huang, Chih-Yang ^{[12
,13
,14
]}

机构：

[1] I Shou Univ, Sch Chinese Med Postbaccalaureate, Kaohsiung 84001, Taiwan

[2] E DA Hosp, Chinese Med Dept, Kaohsiung 82445, Taiwan

[3] 1PT Biotechnol Co Ltd, Taichung 433, Taiwan

[4] China Med Univ, Res Ctr Chinese Med & Acupuncture, Taichung 40402, Taiwan

[5] China Med Univ Hosp, Dept Chinese Med, Taichung 40447, Taiwan

[6] China Med Univ, Coll Chinese Med, Sch Postbaccalaureate Chinese Med, Taichung 40402, Taiwan

[7] China Med Univ, Dept Biol Sci & Technol, Taichung 40447, Taiwan

[8] Changhua Christian Hosp, Dept Pathol, Changhua 50506, Taiwan

[9] Jen Teh Jr Coll Med Nursing & Management, Dept Med Technol, Miaoli 35665, Taiwan

[10] Bharathiar Univ, Dept Biotechnol, Coimbatore 641046, Tamil Nadu, India

[11] Taipei Med Univ, Coll Med, Sch Med, Dept Surg, Taipei 11042, Taiwan

[12] China Med Univ, Grad Inst Basic Med Sci, Taichung 40402, Taiwan

[13] China Med Univ, Sch Chinese Med, Taichung 40447, Taiwan

[14] Asia Univ, Dept Hlth & Nutr Biotechnol, Taichung 41354, Taiwan

来源：

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES | 2016年 / 17卷 / 04期

关键词：

AOF; D-galactose-induced aging; apoptosis; SIRT1; NF-KAPPA-B; OXIDATIVE STRESS; CARDIOPROTECTIVE SUR2A; SIGNAL-TRANSDUCTION; PROTOCATECHUIC ACID; ADVANCED GLYCATION; CARDIAC APOPTOSIS; MYOCYTE APOPTOSIS; IMMUNE-SYSTEM; CELL-SURVIVAL;

D O I：

10.3390/ijms17040466

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Aging, a natural biological/physiological phenomenon, is accelerated by reactive oxygen species (ROS) accumulation and identified by a progressive decrease in physiological function. Several studies have shown a positive relationship between aging and chronic heart failure (HF). Cardiac apoptosis was found in age-related diseases. We used a traditional Chinese medicine, Alpinate Oxyphyllae Fructus (AOF), to evaluate its effect on cardiac anti-apoptosis and pro-survival. Male eight-week-old Sprague-Dawley (SD) rats were segregated into five groups: normal control group (NC), D-Galactose-Induced aging group (Aging), and AOF of 50 (AL (AOF low)), 100 (AM (AOF medium)), 150 (AH (AOF high)) mg/kg/day. After eight weeks, hearts were measured by an Hematoxylin-Eosin (H&E) stain, Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL)-assays and Western blotting. The experimental results show that the cardiomyocyte apoptotic pathway protein expression increased in the D-Galactose-Induced aging groups, with dose-dependent inhibition in the AOF treatment group (AL, AM, and AH). Moreover, the expression of the pro-survival p-Akt (protein kinase B (Akt)), Bcl-2 (B-cell lymphoma 2), anti-apoptotic protein (Bcl-xL) protein decreased significantly in the D-Galactose-induced aging group, with increased performance in the AOF treatment group with levels of p-IGFIR and p-PI3K (Phosphatidylinositol-3' kinase (PI3K)) to increase by dosage and compensatory performance. On the other hand, the protein of the Sirtuin 1 (SIRT1) pathway expression decreased in the aging groups and showed improvement in the AOF treatment group. Our results suggest that AOF strongly works against ROS-induced aging heart problems.

引用

页数：19

共 121 条

[1]

Mild hypoxia in vivo regulates cardioprotective SUR2A: A role for Akt and LDH [J].