The mechanism of vitexin-4''-O-glucoside protecting ECV-304 cells against tertbutyl hydroperoxide induced injury

被引：9

作者：

Li, Hai-bo ^{[1
]}

Ying, Xi-xiang ^{[2
]}

Lu, Jia ^{[2
]}

机构：

[1] Liaoning Univ TCM, Sch Basic Med Sci, Shenyang 110032, Peoples R China

[2] Liaoning Univ Tradit Chinese Med, Sch Pharm, Dalian 116600, Liaoning, Peoples R China

来源：

NATURAL PRODUCT RESEARCH | 2010年 / 24卷 / 18期

关键词：

vitexin-4''-O-glucoside; ECV-304; cell; antioxidation; tertbutyl hydroperoxide;

D O I：

10.1080/14786410902853847

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The aim of this article is to investigate the mechanism of vitexin-4''-O-glucoside (VOG) protecting ECV-304 cells against tertbutyl hydroperoxide (TBHP)-induced injury. ECV-304 cell viability was measured by MTT assay. Apoptosis was determined by terminal deoxynucleotidyl transferase-mediated dUTP nick end-labelling (TUNEL) assay. Cellular morphological changes were observed using phase contrast microscopy. The change of relative mitochondrial transmembrane potential in the ECV-304 cells was analysed with rhodamine 123 staining. Lipid peroxidation was measured by the HPLC method. The results showed that 128 mu mol L-1 VOG could effectively protect ECV-304 cells against cytotoxicity induced by TBHP. VOG protected TBHP-treated ECV-304 cells from death, significantly decreased MDA production, and increased superoxide dismutase (SOD) activity and mitochondrial membrane potential (). Taken together, VOG protects against TBHP-induced ECV-304 cell injury partially through resuming mitochondrial function.

引用

页码：1695 / 1703

页数：9

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