The isolation and sequence of canine interleukin-8 receptor

A cDNA corresponding to canine IL-8 receptor has been cloned and sequenced. The cDNA was synthesized using RT-PCR, with oligonucleotide primers designed from conserved regions of published IL-8 receptors. The 5'-end was cloned by 5'-RACE and the 3'-end was cloned by 3'-RACE. The cDNA encodes a predicted full length IL-8r protein of 356 amino acids. At the nucleic acid level, the canine cDNA shows 83.9%, 82.4%/78.8%, 81.5%/78%, 81.4%/77.7%, 77.8% and 77.3%/71.9% identity to published sequences of bovine, human, gorilla, rabbit, mouse and rat IL8RB/IL8RA, respectively. The derived protein from the cDNA sequences shows 75.3%/70.3%,, 75.3%/70.1%, 74.8%/69.4%, 70%/59%, and 69.7% identity to that of human, rabbit, gorilla, rat and mouse IL8RB/IL8RA homolog.