Molecular characterization of potato virus Y-N isolates by PCR-RFLP - Differentiation of PVYN isolates by PCR-RFLP

被引：50

作者：

Glais, L ^{[1
]}

Kerlan, C ^{[1
]}

Tribodet, M ^{[1
]}

Tordo, VMJ ^{[1
]}

Robaglia, C ^{[1
]}

AstierManifacier, S ^{[1
]}

机构：

[1] INRA, PATHOL VEGETALE STN, F-78026 VERSAILLES, FRANCE

来源：

EUROPEAN JOURNAL OF PLANT PATHOLOGY | 1996年 / 102卷 / 07期

关键词：

PVY; PVYNTN; PTNRD; genome; RNA; polymorphism;

D O I：

10.1007/BF01877246

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Based on the sequence polymorphism in the 5' terminal part of the viral genome, a range of PVYN isolates were characterized by polymerase chain reaction (PCR) followed by restriction fragment length polymorphism (RFLP). Three pairs of primers selected in the 5' non-translated and P1 protein region were tested. Two of them yielded PCR products of about 1Kb from all isolates tested. Restriction analysis of the PCR products gave two distinct electrophoretic patterns, whichever of the three enzymes was used. In this way, the 18 isolates were separated into two easily identifiable subgroups. All tuber necrosing isolates (PVYNTN) were clustered in the same subgroup.

引用

页码：655 / 662

页数：8

共 33 条

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[Anonymous], PHYTOPATH POLONICA

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