Signalling via MHC class II molecules modifies the composition of GEMs in APC

被引：27

作者：

Setterblad, N ^{[1
]}

Becart, S ^{[1
]}

Charron, D ^{[1
]}

Mooney, N ^{[1
]}

机构：

[1] Inst Biomed Cordeliers, INSERM, U396, F-75006 Paris, France

来源：

SCANDINAVIAN JOURNAL OF IMMUNOLOGY | 2001年 / 54卷 / 1-2期

关键词：

D O I：

10.1046/j.1365-3083.2001.00969.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Major histocompatibility complex (MHC) class II molecules are responsible for peptide presentation to helper T lymphocytes and as such play an essential role in the immune response. These molecules transmit intracellular signals leading to diverse consequences in B lymphocytes including proliferation and apoptosis. Recent studies have revealed that glycolipid enriched membrane microdomains (GEMs) behave as signalling platforms for a variety of lymphocyte receptors. We have quantified human leucocyte antigen (HLA)-DR molecules localized in GEMs in human B lymphocytes. Use of a model imitating the interaction of HLA-DR with a T-cell receptor (TCR) modified the constituents of the HLA-DR-enriched GEMs. Confocal microscopy demonstrated a recruitment of HLA-DR and the ganglioside GM1 at the site of HLA-DR interaction with the stimulating ligand. Moreover, cholesterol depletion efficiently impaired this recruitment. Go-localizing proteins detected in HLA-DR-enriched GEMs include protein kinase C (PKC)-delta and actin. These data reveal that MHC class II antigens are localized in GEMs in mature human B lymphocytes and indicates that the formation of the immunological synapse regulates the composition of HLA-DR enriched GEMs in the antigen presenting cell (APC).

引用

页码：87 / 92

页数：6

共 16 条

[1] Concentration of MHC class II molecules in lipid rafts facilitates antigen presentation [J].

Anderson, HA ;

Hiltbold, EM ;

Roche, PA .

NATURE IMMUNOLOGY, 2000, 1 (02) :156-162

[2] Major histocompatibility complex class II-transfected tumor cells present endogenous antigen and are potent inducers of tumor-specific immunity [J].

Armstrong, TD ;

Clements, VK ;

Martin, BK ;

Ting, JPY ;

OstrandRosenberg, S .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (13) :6886-6891

[3]

BRICKGHANNAM C, 1991, J BIOL CHEM, V266, P24169

[4] Functions of lipid rafts in biological membranes [J].

Brown, DA ;

London, E .

ANNUAL REVIEW OF CELL AND DEVELOPMENTAL BIOLOGY, 1998, 14 :111-136

[5] IA BINDING LIGANDS AND CAMP STIMULATE NUCLEAR TRANSLOCATION OF PKC IN LYMPHOCYTES-B [J].

CAMBIER, JC ;

NEWELL, MK ;

JUSTEMENT, LB ;

MCGUIRE, JC ;

LEACH, KL ;

CHEN, ZZ .

NATURE, 1987, 327 (6123) :629-632

[6] THE CYTOPLASMIC AND TRANSMEMBRANE DOMAINS OF MHC CLASS-II BETA-CHAINS DELIVER DISTINCT SIGNALS REQUIRED FOR MHC CLASS II-MEDIATED B-CELL ACTIVATION [J].

HARTON, JA ;

VANHAGEN, AE ;

BISHOP, GA .

IMMUNITY, 1995, 3 (03) :349-358

[7] Intracellular phosphotyrosine induction by major histocompatibility complex class II requires co-aggregation with membrane rafts [J].

Huby, RDJ ;

Dearman, RJ ;

Kimber, I .

JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (32) :22591-22596

[8] Engagement of T cell receptor triggers its recruitment to low-density detergent-insoluble membrane domains [J].

Montixi, C ;

Langlet, C ;

Bernard, AM ;

Thimonier, J ;

Dubois, C ;

Wurbel, MA ;

Chauvin, JP ;

Pierres, M ;

He, HT .

EMBO JOURNAL, 1998, 17 (18) :5334-5348

[9] SIGNALING THROUGH THE MHC CLASS-II CYTOPLASMIC DOMAIN IS REQUIRED FOR ANTIGEN PRESENTATION AND INDUCES B7 EXPRESSION [J].

NABAVI, N ;

FREEMAN, GJ ;

GAULT, A ;

GODFREY, D ;

NADLER, LM ;

GLIMCHER, LH .

NATURE, 1992, 360 (6401) :266-268

[10]

RASBAND W, SCION IMAGE

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