Circulating human antisperm antibodies recognize prostasomes

被引:16
作者
Allegrucci, C
Ronquist, G [1 ]
Nilsson, BO
Carlsson, L
Lundqvist, M
Minelli, A
Larsson, A
机构
[1] Univ Uppsala Hosp, Dept Med Sci, S-75185 Uppsala, Sweden
[2] Univ Perugia, Dipartimento Biol Cellulare & Mol, I-06100 Perugia, Italy
[3] Univ Uppsala Hosp, Dept Obstet & Gynecol, Uppsala, Sweden
[4] Ctr Biomed, Dept Med Cell Biol, Uppsala, Sweden
来源
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY | 2001年 / 46卷 / 03期
关键词
antisperm antibodies (ASA); complement activation; infertility; prostasome; sperm agglutination;
D O I
10.1034/j.1600-0897.2001.d01-4.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
PROBLEM: The presence of naturally occurring antisperm antibodies (ASA) is a well-known cause of infertility in men and women, but the antigens for these antibodies are usually poorly characterized. Prostasomes, organelles secreted by human prostatic acinar cells and expelled into the seminal plasma at ejaculation, can adhere to sperm cells. Thus, we have examined whether prostasomes could be an antigen for ASA. METHOD OF STUDY: We have studied the reactivity of chicken antiprostasome antibodies with sperm cells in an agglutination test and conversely the reactivity of serum positive for ASA from 20 infertile patients, with spermatozoa using flow cytometry and with purified prostasomes using enzyme-linked immunosorbent assay. RESULTS: The chicken antiprostasome antibody caused agglutination of sperm cells similarly to the agglutination observed with patients' sera. All of these patients' sera contained IgG antibodies against prostasomes. CONCLUSIONS: The high percentage of patients with antiprostasome antibodies in this study shows that prostasomes could be one of the major targets for ASA.
引用
收藏
页码:211 / 219
页数:9
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