Genotyping with TaqMAMA

被引：72

作者：

Li, BH

Kadura, I

Fu, DJ

Watson, DE

机构：

[1] Lilly Res Labs, Funct Genomics Grp, Greenfield, IN 46140 USA

[2] Lilly Res Labs, Dept Lead Optimizat Toxicol, Greenfield, IN 46140 USA

来源：

GENOMICS | 2004年 / 83卷 / 02期

关键词：

D O I：

10.1016/j.ygeno.2003.08.005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

TaqMAMA combines the quantitative strengths of TaqMan with the allele-specific PCR of MAMA. In this article we develop TaqMAMA as a technique for screening human DNA samples for known genetic polymorphisms. In the first set of experiments, plasmids that model all types of genetic polymorphisms were used to understand the relationship between TaqMAMA primer/template mismatches and their strength of allelic discrimination. These data can be used to improve allelic discrimination of other primer extension genotyping methodologies through directed use of nucleotide mismatches. We used the data to derive a guide for TaqMAMA primer design and DNA strand selection for TaqMAMA genotyping assays. The guide was then used to develop assays for 11 known and novel human genetic polymorphisms. Genotypes were assigned quickly and accurately in all cases. TaqMAMA genotyping assays require minimal development time, have a high probability of success, produce reliable data that are straightforward to analyze, and are very cost-competitive. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：311 / 320

页数：10

共 14 条

[1] Use of real-time gene-specific polymerase chain reaction to measure RNA expression of three family members of rat cytochrome P450 4A [J].