Protein oxidation markers in women with and without gestational diabetes mellitus: A possible relation with paraoxonase activity

被引:53
作者
Gelisgen, Remise [2 ]
Genc, Habibe [2 ]
Kayali, Refik [3 ]
Oncul, Mahmut [1 ]
Benian, Ali [1 ]
Guralp, Onur [1 ]
Uludag, Seyfettin [1 ]
Cakatay, Ufuk [4 ]
Albayrak, Mustafa [5 ]
Uzun, Hafize [2 ]
机构
[1] Istanbul Univ, Cerrahpasa Tip Fak, Kadin Hastaliklari & Dogum Anabilim Dali, TR-34980 Istanbul, Turkey
[2] Istanbul Univ, Cerrahpasa Med Fac, Dept Biochem, TR-34980 Istanbul, Turkey
[3] Istanbul Univ, Cerrahpasa Med Fac, Dept Pediat, Biochem Lab, TR-34980 Istanbul, Turkey
[4] Istanbul Univ, Istanbul Fac Med, Cent Lab Clin Biochem, TR-34980 Istanbul, Turkey
[5] Duzce Univ, Dept Obstet & Gynecol, Duzce, Turkey
关键词
Gestational diabetes; Protein carbonyl; Paraoxonase; Protein hydroperoxides; Nitrotyrosine; SERUM-LIPIDS; STRESS; PLASMA; DAMAGE; LIPOPROTEINS; PRODUCTS; NITROTYROSINE; BIOCHEMISTRY; PARAMETERS; TYROSINE;
D O I
10.1016/j.diabres.2011.08.001
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
Aims: To clarify the levels of protein oxidation markers such as protein carbonyl (PCO), protein hydroperoxides (P-OOH), advanced oxidation protein products (AOPP) and nitrotyrosine (NT), as well as antioxidative enzymes such as paraoxonase (PON-1) in women with and without gestational diabetes mellitus (GDM). Methods: The study was conducted on 23 women with GDM and 22 women without GDM. The levels of the P-OOH, AOPP, and PON-1 were determined by colorimetric methods; whereas NT and PCO levels were measured by ELISA. Results: The concentrations of protein oxidation markers were significantly increased and PON1 activity was significantly decreased in GDM group compared to those of normal pregnant women. The control group showed a significant negative correlation between PON-1 and PCO (r = -0.451, p = 0.027); whereas in GDM group, there was a significant positive correlation between P-OOH and HbA1c (r = 0.89, p = 0.001). There was no significant correlation between AOPP, PON-1, P-OOH, PCO, and HbA1c in either group. Conclusions: There is evidence of a possible association between protein oxidation and decreased PON1 activity in GDM. The increase in protein oxidation parameters in the GDM group leading to decreased PON1 activity might, we think, create a predisposition for clinical complications in GDM group. (C) 2011 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:404 / 409
页数:6
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