Enteric bacteria of field-collected Colorado potato beetle larvae inhibit growth of the entomopathogens Photorhabdus temperata and Beauveria bassiana

被引：27

作者：

Blackburn, Michael B. ^{[1
]}

Gundersen-Rindal, Dawn E. ^{[1
]}

Weber, Donald C. ^{[1
]}

Martin, Phyllis A. W. ^{[1
]}

Farrar, Robert R., Jr. ^{[1
]}

机构：

[1] Agr Res Serv, Invas Insect Biocontrol & Behav Lab, Henry A Wallace Beltsville Agr Res Ctr, USDA, Beltsville, MD 20705 USA

来源：

BIOLOGICAL CONTROL | 2008年 / 46卷 / 03期

关键词：

colorado potato beetle; Leptinotarsa decemlineata; Heterorhabditis marelatus; Photorhabdus temperata; Beauveria bassiana;

D O I：

10.1016/j.biocontrol.2008.05.005

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The nematode Heterorhabditis marelatus fails to reproduce in the Colorado potato beetle, Leptinotarsa decemlineata, possibly due to interference from the enteric bacteria of the beetle. Specifically, the enteric bacteria inhibit the growth of Photorhabdus temperata, the enteric symbiont of the nematode, in vitro. However, previous work was based on a laboratory culture of L decemlineata, and we wished to determine if similar bacteria were present in the field. Therefore, we cultured the enteric bacteria of fourth-instar larvae collected from the field at two locations in Maryland and Virginia. Representatives of the genera Pantoea, Enterobacter, Pseudomonas, Acinetobacter, Serratia, Stenotrophomonas, Curtobacterium, Bacillus, Lactococcus and Enterococcus were identified by sequencing of their 16S rDNA. Isolates belonging to the genera Pantoea, Enterobacter, Pseudomonas, Serratia and Bacillus inhibited the growth of P. temperata. A number of these isolates also inhibited the entomopathogenic fungus Beauveria bassiana in vitro. Published by Elsevier Inc.

引用

页码：434 / 441

页数：8

共 31 条

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