The Arabidopsis nitrate transporter AtNRT2.1 is targeted to the root plasma membrane

被引：29

作者：

Chopin, Franck

Wirth, Judith

Dorbe, Marie-France

Lejay, Laurence

Krapp, Anne

Gojon, Alain

Daniel-Vedele, Francoise

机构：

[1] INRA, IJPB, Unite Nutr Azotee Plantes, F-78026 Versailles, France

[2] INRA, UM2, Agro M, CNRS,UMR 5004,Inst Biol Integrat Plantes, F-34060 Montpellier 1, France

来源：

PLANT PHYSIOLOGY AND BIOCHEMISTRY | 2007年 / 45卷 / 08期

关键词：

nitrate transporter; Arabidopsis thaliana; GFP confocal imaging; antibodies;

D O I：

10.1016/j.plaphy.2007.04.007

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Arabidopsis AtNRT2.1 protein is the best characterized high affinity nitrate transporter in higher plants. However, nothing is known about its sub-cellular localization. In this work, we used GFP imaging to follow the targeting of the AtNRT2.1 protein to the different cell membranes. A polyclonal antibody was also raised against a peptide derived from the AtNRT2.1 sequence. Comparison of wild type and mutant plant extracts showed that this antibody recognized specifically the AtNRT2.1 protein. Microsomal membranes were fractionated on sucrose gradients and immunological detections were performed on the different fractions. Altogether, our results demonstrate that the AtNRT2.1 protein is located in the plasma membrane of the root cells. (c) 2007 Elsevier Masson SAS. All rights reserved.

引用

页码：630 / 635

页数：6

共 38 条

[1] Major alterations of the regulation of root NO3- uptake are associated with the mutation of Nrt2.1 and Nrt2.2 genes in arabidopsis [J].