Valproic acid modulates NCAM polysialylation and polysialyltransferase mRNA expression in human tumor cells

被引：28

作者：

Beecken, WD ^{[1
]}

Engl, T ^{[1
]}

Ogbomo, H ^{[1
]}

Relja, B ^{[1
]}

Cinatl, J ^{[1
]}

Bereiter-Hahn, E ^{[1
]}

Oppermann, E ^{[1
]}

Jonas, D ^{[1
]}

Blaheta, RA ^{[1
]}

机构：

[1] Univ Frankfurt, Zentrum Chirurgie, Klin Urol & Kinderurol Wissenschaft, D-60590 Frankfurt, Germany

来源：

INTERNATIONAL IMMUNOPHARMACOLOGY | 2005年 / 5卷 / 04期

关键词：

valproic acid; PSA-NCAM; ST8SiaIV; ST8SiaII; tumor dissemination;

D O I：

10.1016/j.intimp.2004.12.009

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Polysialic acid (PSA) is a dynamically regulated carbohydrate modification of the neural cell adhesion molecule NCAM, which has been linked to cancer development and dissemination. Two enzymes, the polysialyltransferases ST8SiaIV and ST8SiaII, are known to be involved in the polysialylation of NCAM. The antiepileptic drug valproic acid (VPA) is associated with anti-cancer activity. In this study, VIA blocked the adhesion of several neuroectodermal tumor cell lines to human umbilical vein endothelial cells. Furthermore, VIA induced intracellular PSA accumulation and enhanced expression of PSA-NCAM on the cell surface. Using a semiquantitative RT-PCR strategy, VIA was shown to up-regulate ST8SiaIV mRNA, whereas ST8SiaII mRNA was down-regulated by this compound. Our data indicate that increased expression of ST8SiaIV enables accelerated polysialylation of NCAM, which might be coupled to a loss of adhesive functions of tumor cells. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：757 / 769

页数：13

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