Detection of an isoform of α1-antitrypsin in serum samples from foals with gastric ulcers

被引：8

作者：

Taharaguchi, S.

Nagano, A.

Okai, K.

Miyasho, T.

Kuwano, M.

Taniyama, H.

Yokota, H. ^{[1
]}

机构：

[1] Rakuno Gakuen Univ, Dept Veterinary Biochem, Ebetsu, Hokkaido 0698501, Japan

[2] Rakuno Gakuen Univ, Sch Vet Med, Dept Veterinary Pathol, Ebetsu, Hokkaido 0698501, Japan

[3] Japan Racing Assoc, Equine Res Inst, Utsunomiya, Tochigi 3200856, Japan

[4] Cent Vet Clin Ctr, Niikappu, Hokkaido 0592403, Japan

来源：

VETERINARY RECORD | 2007年 / 161卷 / 10期

关键词：

D O I：

10.1136/vr.161.10.338

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The objective of this study was to find serum indicators of gastric ulcers in foals. By using two-dimensional electrophoresis of serum proteins, three distinct spots were detected in samples from foals with gastric ulcers detected endoscopically. One of them appeared with high frequency and was identified by partial digestion with trypsin and subsequent nano-electrospray ionisation-tandem mass spectrometry (nanoESI-MS/MS) analysis as an alpha(1)-antitrypsin. Western blot analysis, using an antibody against human alpha(1)-antitrypsin, revealed at least two bands, of molecular weight 58 kDa and 55 kDa, in the sera. The 55 kDa band was detected in 44 of 47 serum samples from foals with gastric ulcers, but in only three of 22 serum samples from healthy foals.

引用

页码：338 / 342

页数：7

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