Organization of the mouse Zjhx1b gene encoding the two-handed zinc finger repressor Smad-interacting protein-1

被引：32

作者：

Nelles, L

Van de Putte, T

van Grunsven, L

Huylebroeck, D

Verschueren, K

机构：

[1] Katholieke Univ Leuven VIB, Dept Dev Biol, B-3000 Louvain, Belgium

[2] Katholieke Univ Leuven, Lab Mol Biol Celgen, B-3000 Louvain, Belgium

来源：

GENOMICS | 2003年 / 82卷 / 04期

关键词：

SIP1; Zfhxlb; zinc finger homeobox 1b protein; Hirschsprung disease; Mowat-Wilson syndrome; gene expression; promoter regions; 5 ' flanking sequence; antisense RNA;

D O I：

10.1016/S0888-7543(03)00169-1

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

SIP], a member of the deltaEF1 family of two-handed zinc finger transcriptional repressors, has been identified as a Smad-binding protein. Recently, mutations in the human SIPI gene (ZFHX1B) have been implicated in Hirschsprung disease. Here we document extensively the structure and transcriptional pattern of the mouse SIP1 gene (Zfhx1b) and compare it to homologues from other species. The overall structure of Zfhx1b is highly similar to that of the deltaEF1 gene (Zfhx1a), confirming their close evolutionary relationship. In contrast to Zfhx1a, the 5' untranslated region of the SIP1-encoding mouse gene is very complex and includes several alternative exons. The corresponding 5'-UTR splicing pattern seems to be conserved between species and suggests a role in its transcriptional and/or translational regulation. The gene also codes for an antisense transcript that is highly conserved between human and mouse. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：460 / 469

页数：10

共 23 条

[1] Large-scale deletions and SMADIP1 truncating mutations in syndromic hirschsprung disease with involvement of midline structures [J].