Determination of Chloramphenicol Residues in Foods by ELISA and LC-MS/MS Coupled with Molecularly Imprinted Solid Phase Extraction

被引：11

作者：

Shi, Xizhi ^{[1
]}

Song, Suquan ^{[2
,3
]}

Sun, Aili

Li, Dexiang

Wu, Aibo ^{[2
]}

Zhang, Dabing ^{[2
]}

机构：

[1] Ningbo Univ, Fac Life Sci & Biotechnol, Key Lab Appl Marine Biotechnol, Minist Educ, Ningbo 315211, Zhejiang, Peoples R China

[2] Shanghai Jiao Tong Univ, Bor Luh Food Safety Ctr, Coll Life Sci & Biotechnol, Shanghai 200030, Peoples R China

[3] Shanghai Acad Agr Sci, Inst Agri Food Stand & Testing Technol, Shanghai, Peoples R China

来源：

ANALYTICAL LETTERS | 2010年 / 43卷 / 17期

关键词：

Chloramphenicol; Foods; Molecularly imprinted polymer microspheres; TRACE ANALYSIS; ESI-MS/MS; HONEY; POLYMER; MILK; CHROMATOGRAPHY; SAMPLES; WATER; CONFIRMATION; COMBINATION;

D O I：

10.1080/00032711003763616

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The combination of molecularly imprinted solid-phase extraction (MISPE) with ELISA and LC-MS/MS was developed for the detection of chloramphenicol (CAP) in honey samples. Significant recoveries of 99.1 +/- 7.1 and 98.8 +/- 8.2% were obtained for intra- and inter-assay determination by ELISA determination, respectively. The limit of detection of CAP was 0.034gkg-1 and the limit of quantification was 0.046gkg-1. Determination and validation of CAP by using LC-MS/MS were performed following the same extraction and purification process as for the ELISA. The results demonstrated that the CAP samples purified by using MISPE were simultaneously applicable to analysis by ELISA and LC-MS/MS.

引用

页码：2798 / 2807

页数：10

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