Comparison of different particles and methods for magnetic isolation of circulating tumor cells

被引:91
作者
Sieben, S
Bergemann, C
Lübbe, A
Brockmann, B
Rescheleit, D
机构
[1] Labsoft GmbH, D-06108 Halle, Germany
[2] Chemicell GmbH, D-10777 Berlin, Germany
[3] Cecilien Klin, D-33175 Bad Lippspringe, Germany
[4] Humaine Klin, D-01326 Dresden, Germany
关键词
circulating tumor cells; epithelial tumor cells; immunomagnetic separation; magnetic beads; microspheres;
D O I
10.1016/S0304-8853(00)01248-8
中图分类号
T [工业技术];
学科分类号
08 ;
摘要
A more effective method for tumor cell separation from peripheral blood was established. The results of optimized magnetic particles verified by analyzing yield, purity and viability of isolated epithelial tumor cells were compared with a commercial kit for immunomagnetic cell separation. Porous silica particles of 230 nm were found to give best recovery rates and high viability of extracted cells. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:175 / 179
页数:5
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