Astrocytes expressing glutamic acid decarboxylase GAD67 directed by the glial fibrillary acidic protein promoter were shown to provide enhanced protection of PC12 cells from H2O2 treatment and serum deprivation in the presence of glutamate. In addition, they protected non-differentiated, but not differentiated, embryonic rat cortical neurons from glutamate toxicity. Glutamic acid decarboxylase (GAD)expressing astrocytes showed increased glutathione synthesis and release compared to control astrocytes. These changes were due to GAD transgene expression, as transient expression of a GAD antisense plasmid resulted in partial suppression of the increase in glutathione release. In addition to the previously demonstrated increases in NADH and ATP levels and lactate release, GAD-expressing astrocytes show increased antioxidant activity, explaining their ability to protect neurons from various injuries.
机构:
HOP BROUSSAIS, INSERM, UNITE RECH METAB & DIFFERENCIAT CELLULES CULTURE U, F-75674 PARIS 14, FRANCEHOP BROUSSAIS, INSERM, UNITE RECH METAB & DIFFERENCIAT CELLULES CULTURE U, F-75674 PARIS 14, FRANCE
ALLIOT, F
PESSAC, B
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机构:
HOP BROUSSAIS, INSERM, UNITE RECH METAB & DIFFERENCIAT CELLULES CULTURE U, F-75674 PARIS 14, FRANCEHOP BROUSSAIS, INSERM, UNITE RECH METAB & DIFFERENCIAT CELLULES CULTURE U, F-75674 PARIS 14, FRANCE
机构:
Cornell Univ, Weill Med Coll, Dept Neurol & Neurosurg, New York, NY 10021 USACornell Univ, Weill Med Coll, Dept Neurol & Neurosurg, New York, NY 10021 USA
机构:
HOP BROUSSAIS, INSERM, UNITE RECH METAB & DIFFERENCIAT CELLULES CULTURE U, F-75674 PARIS 14, FRANCEHOP BROUSSAIS, INSERM, UNITE RECH METAB & DIFFERENCIAT CELLULES CULTURE U, F-75674 PARIS 14, FRANCE
ALLIOT, F
PESSAC, B
论文数: 0引用数: 0
h-index: 0
机构:
HOP BROUSSAIS, INSERM, UNITE RECH METAB & DIFFERENCIAT CELLULES CULTURE U, F-75674 PARIS 14, FRANCEHOP BROUSSAIS, INSERM, UNITE RECH METAB & DIFFERENCIAT CELLULES CULTURE U, F-75674 PARIS 14, FRANCE
机构:
Cornell Univ, Weill Med Coll, Dept Neurol & Neurosurg, New York, NY 10021 USACornell Univ, Weill Med Coll, Dept Neurol & Neurosurg, New York, NY 10021 USA