FT-IR study of heterologous protein expression in recombinant Escherichia coli strains

被引：39

作者：

Ami, D

Bonecchi, L

Calì, S

Orsini, G

Tonon, G

Doglia, SM

机构：

[1] Univ Milano Bicocca, Dept Biosci & Biotechnol, INFM, UdR Milano Bicocca, I-20126 Milan, Italy

[2] Keryos Spa, Div Biotechnol, I-20097 San Donato Milanese, Italy

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS | 2003年 / 1624卷 / 1-3期

关键词：

inclusion body; FT-IR microspectroscopy; Escherichia coli; expression of heterologous protein; interferon alpha;

D O I：

10.1016/j.bbagen.2003.09.008

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two recombinant Escherichia coli strains expressing different levels of an interferon fusion protein as inclusion bodies have been studied by Fourier transform infrared (FT-IR) microspectroscopy. A marker band at 1628 cm(-1) allowed monitoring of the protein expression by direct analysis of cell pellets in a rapid, non-invasive and quantitative way. The results demonstrate that FT-IR microspectroscopy is a technique of potential biotechnological. interest for studying inclusion body formation. (C) 2003 Elsevier B.V. All rights reserved.

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页码：6 / 10

页数：5

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