Crucial importance of PKC-β(I) in LFA-I-mediated locomotion of activated T cells

被引:120
作者
Volkov, Y [1 ]
Long, A
McGrath, S
Eidhin, DN
Kelleher, D
机构
[1] Univ Ireland Trinity Coll, Dublin Mol Med Ctr, Dublin 8, Ireland
[2] Univ Ireland Trinity Coll, Dept Clin Med, Dublin 8, Ireland
[3] Royal Coll Surg Ireland, Dept Biochem, Dublin 2, Ireland
关键词
D O I
10.1038/88700
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Crawling T cell locomotion in which activated lymphocyte function-associated antigen I (LFA-I) integrins participate is associated with translocation of the protein kinase C-beta (PKC-beta) isoenzyme to the microtubule cytoskeleton. In normal T cells and T lymphoma cell lines, this type of motility is accompanied by PKC-beta -sensitive cytoskeletal rearrangements and the formation of trailing cell extensions, which are supported by microtubules. Expression of PKC-beta (I) and enhanced green fluorescent protein (EGFP) in nonmotile PKC-beta -deficient T cells restored their locomotory behavior in response to a triggering stimulus delivered via LFA-I and correlated directly with the degree of cell polarization. We have also shown that PKC-beta (I) is a component of the tubulin-enriched LFA-I-cytoskeletal complex assembled upon LFA-I cross-linking. These observations may have physiological equivalents at advanced (post-integrin activation) stages of lymphocyte extravasation.
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页码:508 / 514
页数:7
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