Isolation of bacteriophages from Bartonella vinsonii subsp berkhoffii and the characterization of Pap31 gene sequences from bacterial and phage DNA

被引:34
作者
Maggi, RG [1 ]
Breitschwerdt, EB [1 ]
机构
[1] N Carolina State Univ, Coll Vet Med, Dept Clin Sci, Vector Borne Dis Diagnost Lab, Raleigh, NC 27606 USA
关键词
alpha-proteobacteria; virus; canine; pathogen; phage-associated protein; Bartonella;
D O I
10.1159/000088145
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Bacteriophages enhance bacterial survival, facilitate bacterial adaptation to new environmental conditions, assist in the adaptation to a new host species, and enhance bacterial evasion or inactivation of host defense mechanisms. We describe the detection and purification of a novel tailed bacteriophage from Bartonella vinsonii subsp. berkhoffii, which was previously described as a bacteriophage-negative species. We also compare B. vinsonii subsp. berkhoffi Pap31 bacteriophage gene sequences to B. henselae ( Houston I), and B. quintana ( Fuller) bacteriophage Pap31 sequences. Negative staining electron microscopy of log phase cultures of B. vinsonii subsp. berkhoffii identified bacteriophages, possessing a 50-nm icosahedric head diameter and a 60- to 80-nm contractile tail. Sequence analysis of the bacteriophage Pap31 gene from B. vinsonii subsp. berkhoffii showed three consensus sequences and a 12-bp insertion when compared with Pap31 gene sequences from B. henselae ( Houston I) and B. quintana ( Fuller) bacteriophages. Isolation of B. vinsonii subsp. berkhoffii bacteriophages containing a Pap31 gene suggests that this heme-binding protein gene might play an important role in bacterial virulence through the genetic exchange of DNA within this subspecies. Defining phage-associated genes may also contribute to the enhanced understanding of the evolutionary relationships among members of the genus Bartonella. Copyright (C) 2005 S. Karger AG, Basel.
引用
收藏
页码:44 / 51
页数:8
相关论文
共 70 条
[1]   CAT-SCRATCH DISEASE, BACILLARY ANGIOMATOSIS, AND OTHER INFECTIONS DUE TO ROCHALIMAEA [J].
ADAL, KA ;
COCKERELL, CJ ;
PETRI, WA .
NEW ENGLAND JOURNAL OF MEDICINE, 1994, 330 (21) :1509-1515
[2]   The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae [J].
Alsmark, CM ;
Frank, AC ;
Karlberg, EO ;
Legault, BA ;
Ardell, DH ;
Canbäck, B ;
Eriksson, AS ;
Näslund, AK ;
Handley, SA ;
Huvet, M ;
La Scola, B ;
Holmberg, M ;
Andersson, SGE .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (26) :9716-9721
[3]   BACTERIOPHAGE-LIKE PARTICLE OF ROCHALIMAEA-HENSELAE [J].
ANDERSON, B ;
GOLDSMITH, C ;
JOHNSON, A ;
PADMALAYAM, I ;
BAUMSTARK, B .
MOLECULAR MICROBIOLOGY, 1994, 13 (01) :67-73
[4]   Analysis of a 36-kilodalton protein (PapA) associated with the bacteriophage particle of Bartonella henselae [J].
Anderson, B ;
Scotchlas, D ;
Jones, D ;
Johnson, A ;
Tzianabos, T ;
Baumstark, B .
DNA AND CELL BIOLOGY, 1997, 16 (10) :1223-1229
[5]   Bartonella spp. as emerging human pathogens [J].
Anderson, BE ;
Neuman, MA .
CLINICAL MICROBIOLOGY REVIEWS, 1997, 10 (02) :203-+
[6]   Cat-scratch disease encephalopathy: A cause of status epilepticus in school-aged children [J].
Armengol, CE ;
Hendley, JO .
JOURNAL OF PEDIATRICS, 1999, 134 (05) :635-638
[7]  
AUSUBEL FM, 1997, SHORT PROTOCOLS MOL, P1512
[8]  
AVIDOR B, 2004, 4 INT C BART EM PATH
[9]   Prophage induction and expression of prophage-encoded virulence factors in group A Streptococcus serotype M3 strain MGAS315 [J].
Banks, DJ ;
Lei, BF ;
Musser, JM .
INFECTION AND IMMUNITY, 2003, 71 (12) :7079-7086
[10]   A bacteriophage-like particle from Bartonella bacilliformis [J].
Barbian, KD ;
Minnick, MF .
MICROBIOLOGY-UK, 2000, 146 :599-609