Ecdysteroid antagonists (cucurbitacins) from Physocarpus opulifolius (Rosaceae)

被引：18

作者：

Sarker, SD

Whiting, P

Sik, V

Dinan, L

机构：

[1] Univ Exeter, Dept Biol Sci, Hatherly Labs, Exeter EX4 4PS, Devon, England

[2] Univ Exeter, Dept Chem, Exeter EX4 4QD, Devon, England

来源：

PHYTOCHEMISTRY | 1999年 / 50卷 / 07期

基金：

英国生物技术与生命科学研究理事会;

关键词：

Physocarpus opulifolius; rosaceae; ninebark; cucurbitacin D; 3-epi-isocucurbitacin D cucurbitacin F; ecdysteroid antagonist; chemotaxonomy;

D O I：

10.1016/S0031-9422(98)00653-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Methanolic extracts of seeds of 4 species in the genus Physocarpus antagonise the action of the insect steroid hormone 20-hydroxyecdysone on a Drosophila melanogaster permanent cell line. The active components in the extract of P. opulifolius (ninebark) have been identified as cucurbitacin D, cucurbitacin F and 3-epi-isocucurbitacin D. The potencies of the individual cucurbitacins have been determined as 5 x 10(-7), 8 x 10(-7) and 7x10(-6) M, respectively (versus 5 x 10(-8) M 20-hydroxyecdysone). The distribution of antagonistic activity in plants of P. opulifolius has been assessed and HPLC/bioassay has been used to determine the chromatographic profiles of antagonist activity in parts of the growing plant. (C) 1999 Elsevier Science Ltd. All rights reserved.

引用

页码：1123 / 1128

页数：6

共 28 条

[1] Cytotoxic principles from Chrysosplenium flagelliferum [J].