Cytoplasmic tails of hantavirus glycoproteins interact with the nucleocapsid protein

被引:50
作者
Hepojoki, J. [1 ,2 ]
Strandin, T. [1 ,2 ]
Wang, H. [1 ]
Vapalahti, O. [1 ,3 ]
Vaheri, A. [1 ]
Lankinen, H. [1 ,2 ]
机构
[1] Univ Helsinki, Haartman Inst, Infect Biol Res Program, Dept Virol, FIN-00014 Helsinki, Finland
[2] Univ Helsinki, Haartman Inst, Infect Biol Res Program, Peptide & Prot Lab, FIN-00014 Helsinki, Finland
[3] Univ Helsinki, Fac Vet Med, FIN-00014 Helsinki, Finland
关键词
VIRUS CORE PROTEIN; PUUMALA VIRUS; MEMBRANE-PROTEIN; TULA HANTAVIRUS; INFECTIONS; DOMAIN; OLIGOMERIZATION; REASSORTMENT; EXPRESSION; COMPLEX;
D O I
10.1099/vir.0.021006-0
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Here we characterize the interaction between the glycoproteins (Gn and Gc) and the ribonucleoprotein (RNP) of Puumala virus (PUUV; genus Hantavirus, family Bunyaviridae). The interaction was initially established with native proteins by co-immunoprecipitating PUUV nucleocapsid (N) protein with the glycoprotein complex. Mapping of the interaction sites revealed that the N protein has multiple binding sites in the cytoplasmic tail (CT) of Gn and is also able to bind to the predicted CT of Gc. The importance of Gn- and Gc-CTs to the recognition of RNP was further verified in pull-down assays using soluble peptides with binding capacity to both recombinant N protein and the RNPs of PUUV and Tula virus. Additionally, the N protein of PUUV was demonstrated to interact with peptides of Gn and Gc from a variety of hantavirus species, suggesting a conserved RNP-recognition mechanism within the genus. Based on these and our previous results, we suggest that the complete hetero-oligomeric (Gn-Gc)(4) spike complex of hantaviruses mediates the packaging of RNP into virions.
引用
收藏
页码:2341 / 2350
页数:10
相关论文
共 51 条
[1]   Oligomerization of hantaviral nucleocapsid protein:: charged residues in the N-terminal coiled-coil domain contribute to intermolecular interactions [J].
Alminaite, Agne ;
Backstrom, Vera ;
Vaheri, Antti ;
Plyusnin, Alexander .
JOURNAL OF GENERAL VIROLOGY, 2008, 89 :2167-2174
[2]   The nucleocapsid-binding spike subunit E2 of Semliki Forest virus requires complex formation with the E1 subunit for activity [J].
Barth, BU ;
Garoff, H .
JOURNAL OF VIROLOGY, 1997, 71 (10) :7857-7865
[3]   Mutational Analysis of the Bunyamwera Orthobunyavirus Nucleocapsid Protein Gene [J].
Eifan, Saleh A. ;
Elliott, Richard M. .
JOURNAL OF VIROLOGY, 2009, 83 (21) :11307-11317
[4]  
Elliott R.M., 2000, Virus Taxonomy: Seventh Report of the International Committee on Taxonomy of Viruses, P599
[5]   Emerging viruses: The Bunyaviridae [J].
Elliott, RM .
MOLECULAR MEDICINE, 1997, 3 (09) :572-577
[6]   The Hantavirus Glycoprotein G1 Tail Contains Dual CCHC-type Classical Zinc Fingers [J].
Estrada, D. Fernando ;
Boudreaux, Daniel M. ;
Zhong, Dalian ;
Jeor, Stephen C. St. ;
De Guzman, Roberto N. .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2009, 284 (13) :8645-8651
[7]  
Flint S.J., 2000, PRINCIPLES VIROLOGY
[8]   The SPOT synthesis technique - Synthetic peptide arrays on membrane supports - principles and applications [J].
Frank, R .
JOURNAL OF IMMUNOLOGICAL METHODS, 2002, 267 (01) :13-26
[9]   RNA-BINDING OF RECOMBINANT NUCLEOCAPSID PROTEINS OF HANTAVIRUSES [J].
GOTT, P ;
STOHWASSER, R ;
SCHNITZLER, P ;
DARAI, G ;
BAUTZ, EKF .
VIROLOGY, 1993, 194 (01) :332-337
[10]   Characterization of protein-protein interactions between the nucleocapsid protein and membrane protein of the SARS coronavirus [J].
He, RT ;
Leeson, A ;
Ballantine, M ;
Andonov, A ;
Baker, L ;
Dobie, F ;
Li, Y ;
Bastien, N ;
Feldmann, H ;
Strocher, U ;
Theriault, S ;
Cutts, T ;
Cao, JX ;
Booth, TF ;
Plummer, FA ;
Tyler, S ;
Li, XG .
VIRUS RESEARCH, 2004, 105 (02) :121-125