AmtB-mediated NH3 transport in prokaryotes must be active and as a consequence regulation of transport by GlnK is mandatory to limit futile cycling of NH4+/NH3

被引:43
作者
Boogerd, Fred C. [1 ]
Ma, Hongwu [2 ]
Bruggeman, Frank J.
van Heeswijk, Wally C.
Garcia-Contreras, Rodolfo
Molenaar, Douwe
Krab, Klaas
Westerhoff, Hans V. [3 ]
机构
[1] Vrije Univ Amsterdam, Fac Earth & Life Sci, Netherlands Inst Syst Biol, Sect Mol Cell Physiol,Dept Mol Cell Physiol, NL-1081 HV Amsterdam, Netherlands
[2] Univ Edinburgh, Sch Informat, Edinburgh EH8 9YL, Midlothian, Scotland
[3] Univ Manchester, Manchester Ctr Integrat Syst Biol, Manchester M13 9PL, Lancs, England
基金
英国生物技术与生命科学研究理事会;
关键词
AmtB; Active transport; Futile cycling; GlnK; SIGNAL-TRANSDUCTION PROTEIN; AMMONIUM CHANNEL AMTB; ESCHERICHIA-COLI; GLUTAMATE-DEHYDROGENASE; MOLECULAR-MECHANISMS; PHYSIOLOGICAL-ROLE; CRYSTAL-STRUCTURE; COMPLEX-FORMATION; IN-VIVO; WATER;
D O I
10.1016/j.febslet.2010.11.055
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The nature of the ammonium import into prokaryotes has been controversial. A systems biological approach makes us hypothesize that AmtB-mediated import must be active for intracellular NH4+ concentrations to sustain growth. Revisiting experimental evidence, we find the permeability assays reporting passive NH3 import inconclusive. As an inevitable consequence of the proposed NH4+ transport, outward permeation of NH3 constitutes a futile cycle. We hypothesize that the regulatory protein GlnK is required to fine-tune the active transport of ammonium in order to limit futile cycling whilst enabling an intracellular ammonium level sufficient for the cell's nitrogen requirements. (C) 2010 Federation of European Biochemical Societies. Published by Elsevier B. V. All rights reserved.
引用
收藏
页码:23 / 28
页数:6
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