Peripheral sequences of the Serratia entomophila pADAP virulence-associated region

被引：11

作者：

Hurst, MRH ^{[1
]}

O'Callaghan, M ^{[1
]}

Glare, TR ^{[1
]}

机构：

[1] AgRes, Biocontrol & Biosecur, Lincoln, New Zealand

来源：

PLASMID | 2003年 / 50卷 / 03期

关键词：

pADAP; Serratia entomophila; Costelytra zealandica; sepA; sepB; sepC; IS66; IS91; sef;

D O I：

10.1016/S0147-619X(03)00062-3

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Some strains of the Enterobacteriaceae Serratia entomophila and Serratia proteamaculans cause amber disease in the grass grub, Costelytra zealandica (Coleoptera: Scarabaeidae), an important pasture pest in New Zealand. The genes responsible for this disease reside on a large, 155-kb plasmid designated amber disease-associated plasmid (pADAP). Herein, we report the DNA sequencing of approximately 50 kb upstream and 10 kb downstream of the virulence-encoding region. Based on similarity with proteins in the current databases, and potential ribosome-binding sites, 63 potential ORFs were determined. Eleven of these ORFs belong to a type IV pilus cluster (pilL-V) and a further eight have similarities to the translated products of the plasmid transfer traH-N genes of the plasmid R64. In addition, a degenerate 785-nt direct repeat flanks a 44.7-kb region with the potential to encode three Bacillus subtilis Yee-type proteins, a fimbrial gene cluster, the sep virulence-associated genes and several remnant IS elements. (C) 2003 Elsevier Inc. All rights reserved.

引用

页码：213 / 229

页数：17

共 83 条

[1] Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].