Facile reversed-phase HPLC resolution and quantitative determination of aristolochic acid and aristolactam analogues in traditional Chinese medicine

被引:18
作者
Jon, JH
Chen, SS
Wu, TS
机构
[1] Natl Cheng Kung Univ, Dept Chem, Tainan 701, Taiwan
[2] Chia Nan Univ Pharm & Sci, Dept Food Hlth, Jente, Taiwan
[3] Natl Chiayi Univ, Dept Appl Chem, Chiayi, Taiwan
关键词
aristolochic acid; aristolactam analogs; RP-HPLC; traditional Chinese medicine; resolution; quantitative determination;
D O I
10.1081/JLC-120025422
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
A facile reversed-phase HPLC method for simultaneous resolution and quantitative determination of 17 analogues of aristolochic acid (AA) and aristolactam (AL) under gradient elution, with solvent mixture of sodium acetate and acetonitrile, is described. The retention scale of analytes examined in this study is found to be dependent on the pH of mobile phase and highly relevant to the presence of functional groups on analytes as well, under the same chromatographic conditions. Analytes with high polarity (i.e., many functional groups) elutes early. Increasing the pH of mobile phase decreases the retention time of AA analogues, however, it increases that of AL analogues slightly At higher pH (>5.0), the elution reversal between AA and AL is observed and believed to be from the ionization of carboxyl groups on aristolochic acid. Quantitative analysis of Chinese herbs such as Aristolochia heterophylla and Aristolochia fangchi has proven this method to be facile and reliable, which can be considered as fingerprints to identify the origin or species of plant in the genus Aristolochia.
引用
收藏
页码:3057 / 3068
页数:12
相关论文
共 27 条
[1]  
BIALECKI M, 1973, Herba Polonica, V19, P370
[2]  
CATENI F, 1992, FARMACO, V47, P1335
[3]  
Denke A, 1999, ARZNEIMITTEL-FORSCH, V49, P120
[4]  
GORECKI P, 1975, Herba Polonica, V21, P148
[5]   TUMOR INHIBITORS .1. ARISTOLOCHIC ACID, ACTIVE PRINCIPLE OF ARISTOLOCHIA INDICA [J].
KUPCHAN, SM ;
DOSKOTCH, RW .
JOURNAL OF MEDICINAL & PHARMACEUTICAL CHEMISTRY, 1962, 5 (03) :657-&
[6]   High-performance liquid chromatographic determination for aristolochic acid in medicinal plants and slimming products [J].
Lee, TY ;
Wu, ML ;
Deng, JF ;
Hwang, DF .
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES, 2002, 766 (01) :169-174
[7]  
LIU Y, 1990, ZHONGCAOYAO, V21, P15
[8]   Nephropathy caused by Chinese herbs in the Uh [J].
Lord, GM ;
Tagore, R ;
Cook, T ;
Gower, P ;
Pusey, CD .
LANCET, 1999, 354 (9177) :481-482
[9]   Urothelial malignant disease and Chinese herbal nephropathy [J].
Lord, GM ;
Cook, T ;
Arlt, VM ;
Schmeiser, HH ;
Williams, G ;
Pusey, CD .
LANCET, 2001, 358 (9292) :1515-1516
[10]   ISOLATION OF ARISTOLOCHIC ACIDS HASI AND HASII BY PREPARATIVE LIQUID-CHROMATOGRAPHY [J].
MAKUCH, B ;
GAZDA, K ;
CISOWSKI, W .
JOURNAL OF CHROMATOGRAPHY, 1992, 594 (1-2) :145-151