Single-molecule experiments in synthetic biology: An approach to the affinity ranking of DNA-binding peptides

被引：53

作者：

Eckel, R ^{[1
]}

Wilking, SD ^{[1
]}

Becker, A ^{[1
]}

Sewald, N ^{[1
]}

Ros, R ^{[1
]}

Anselmetti, D ^{[1
]}

机构：

[1] Univ Bielefeld, D-33615 Bielefeld, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2005年 / 44卷 / 25期

关键词：

molecular recognition; peptides; point mutations; scanning probe microscopy; single-molecule studies;

D O I：

10.1002/anie.200500152

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

(Figure Presented) Peptide-DNA recognition: The specific binding of peptides to DNA representing a section of the binding sequence of a transcription activator has been demonstrated for the first time at the single-molecule level with AFM (experimental principle, see picture). The results underscore the importance of peptide length and cooperative effects as well as the contribution of single-point mutations on specific binding. © 2005 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：3921 / 3924

页数：4

共 43 条

[1]

[Anonymous], 2001, ANGEW CHEM, V113, P4859

[2] Synthetic biology for nanotechnology [J].