Solvent-based extraction optimisation for efficient ultrasonication-assisted ginsenoside recovery from Panax quinquefolius and P. sikkimensis cell suspension lines

被引：12

作者：

Biswas, Tanya ^{[1
]}

Ajayakumar, P. V. ^{[2
]}

Mathur, Ajay Kumar ^{[1
]}

Mathur, Archana ^{[1
]}

机构：

[1] CSIR, Div Plant Biotechnol, CSIR Cent Inst Med & Aromat Plants, Lucknow 226015, Uttar Pradesh, India

[2] CSIR, Div Analyt Chem, CSIR Cent Inst Med & Aromat Plants, Lucknow 226015, Uttar Pradesh, India

来源：

NATURAL PRODUCT RESEARCH | 2015年 / 29卷 / 13期

关键词：

HPLC; Panax sikkimensis; ultrasonication-assisted extraction; ginsenosides; Panax quinquefolius; HPTLC; AMERICAN GINSENG; ROOT;

D O I：

10.1080/14786419.2015.1024119

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

The present study aims at developing an extraction protocol for efficient ginsenoside recovery from cell suspensions of Panax quinquefolius and P. sikkimensis. Methanol (100%, 70% and 30%), water (40 degrees C, 90 degrees C), water-saturated butanol and butanol-saturated water were compared for their ultrasonication-assisted ginsenoside retrieval efficacy. HPLC and HP-TLC analysis revealed 100% methanol as the best solvent for maximum retrieval of Rb (diol) and Rg (triol) ginsenosides (P. quinquefolius: Rb: 0.189, Rg: 3.163mg/g DW; P. sikkimensis: Rb: 0.245, Rg: 4.073mg/g DW), followed by water (90 degrees C). Methanolic solutions, especially 70%, proved to be significant retrievers of Rg1 (1.812 and 1.327mg/g DW in P. quinquefolius and P. sikkimensis), with poor Re recovery (0.328 and 0.342mg/g DW). Water-saturated butanol also led to significant ginsenoside extraction (72.4% of content extracted by methanol), selectively in P. quinquefolius, with a less than 50% of total content extracted by methanol, in P. sikkimensis.

引用

页码：1256 / 1263

页数：8

共 13 条

[1] Simplified extraction of ginsenosides from American ginseng (Panax quinquefolius L.) for high-performance liquid chromatography-ultraviolet analysis [J].