Elucidating chromatin and nuclear domain architecture with electron spectroscopic imaging

被引：33

作者：

Bazett-Jones, David P. ^{[1
]}

Li, Ren ^{[1
]}

Fussner, Eden ^{[1
]}

Nisman, Rosa ^{[1
]}

Dehghani, Hesam ^{[2
]}

机构：

[1] Hosp Sick Children, Res Inst, Program Genet & Genome Biol, Toronto, ON M5G 1L7, Canada

[2] Ferdowsi Univ Mashhad, Sch Vet Med, Dept Physiol, Mashhad, Iran

来源：

CHROMOSOME RESEARCH | 2008年 / 16卷 / 03期

基金：

加拿大健康研究院; 加拿大自然科学与工程研究理事会;

关键词：

chromatin; correlative microscopy; electron microscopy; heterochromatin; nuclear speckles; nuclear structure; nucleolus; promyelocytic leukemia; transcription;

D O I：

10.1007/s10577-008-1237-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Electron microscopy has been the 'gold standard' of spatial resolution for studying the structure of the cell nucleus. Electron spectroscopic imaging (ESI) offers advantages over conventional transmission electron microscopy by eliminating the need for heavy-atom contrast agents. ESI also provides mass-dependent and element-specific information at high resolution, permitting the distinguishing of structures that are primarily composed of protein, DNA, or RNA. The technique can be applied to understand the structural consequences of epigenetic modifications, such as modified histones, on chromatin fiber morphology. ESI can also be applied to elucidate the multifunctional behavior of subnuclear 'organelles' such as the nucleolus and promyelocytic leukemia nuclear bodies.

引用

页码：397 / 412

页数：16

共 35 条

[1] Efficient supercoiling of DNA by a single condensin complex as revealed by electron spectroscopic imaging [J].

Bazett-Jones, DP ;

Kimura, K ;

Hirano, T .

MOLECULAR CELL, 2002, 9 (06) :1183-1190

[2] Electron spectroscopic imaging of chromatin [J].

Bazett-Jones, DP ;

Hendzel, MJ .

METHODS-A COMPANION TO METHODS IN ENZYMOLOGY, 1999, 17 (02) :188-200

[3] Stoichiometric analysis of protein- and nucleic acid-based structures in the cell nucleus [J].

Bazett-Jones, DP ;

Hendzel, MJ ;

Kruhlak, MJ .

MICRON, 1999, 30 (02) :151-157

[4] SHORT-RANGE DNA LOOPING BY THE XENOPUS HMG-BOX TRANSCRIPTION FACTOR, XUBF [J].

BAZETTJONES, DP ;

LEBLANC, B ;

HERFORT, M ;

MOSS, T .

SCIENCE, 1994, 264 (5162) :1134-1137

[5] Visualization and analysis of unfolded nucleosomes associated with transcribing chromatin [J].

BazettJones, DP ;

Mendez, E ;

Czarnota, GJ ;

Ottensmeyer, FP ;

Allfrey, VG .

NUCLEIC ACIDS RESEARCH, 1996, 24 (02) :321-329

[6] Promyelocytic leukemia (PML) nuclear bodies are protein structures that do not accumulate RNA [J].

Boisvert, FM ;

Hendzel, MJ ;

Bazett-Jones, DP .

JOURNAL OF CELL BIOLOGY, 2000, 148 (02) :283-292

[7] Three-dimensional maps of all chromosomes in human male fibroblast nuclei and prometaphase rosettes [J].

Bolzer, A ;

Kreth, G ;

Solovei, I ;

Koehler, D ;

Saracoglu, K ;

Fauth, C ;

Müller, S ;

Eils, R ;

Cremer, C ;

Speicher, MR ;

Cremer, T .

PLOS BIOLOGY, 2005, 3 (05) :826-842

[8] The spatial organization of human chromosomes within the nuclei of normal and emerin-mutant cells [J].

Boyle, S ;

Gilchrist, S ;

Bridger, JM ;

Mahy, NL ;

Ellis, JA ;

Bickmore, WA .

HUMAN MOLECULAR GENETICS, 2001, 10 (03) :211-219

[9] Protein and DNA requirements for the transcription factor IIIA-induced distortion of the 5 S rRNA gene promoter [J].

Brown, ML ;

Schroth, GP ;

Gottesfeld, JM ;

BazettJones, DP .

JOURNAL OF MOLECULAR BIOLOGY, 1996, 262 (05) :600-614

[10] Chromatin decondensation and nuclear reorganization of the HoxB locus upon induction of transcription [J].

Chambeyron, S ;

Bickmore, WA .

GENES & DEVELOPMENT, 2004, 18 (10) :1119-1130

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