Probing metal-protein interactions using a de novo design approach

被引：56

作者：

Ghosh, D ^{[1
]}

Pecoraro, VL ^{[1
]}

机构：

[1] Univ Michigan, Dept Chem, Ann Arbor, MI 48109 USA

来源：

CURRENT OPINION IN CHEMICAL BIOLOGY | 2005年 / 9卷 / 02期

关键词：

D O I：

10.1016/j.cbpa.2005.02.005

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

De novo design of metalloproteins provides a valuable tool for understanding the structural constraints and functional attributes of natural biological systems using first principles. This review focuses on recent research aimed primarily at probing the subtle interactions between metals and proteins in designed systems. Considerable attention has focussed on redefining novel design methods used in mimicking natural hemeproteins, mononuclear and dinuclear metallopeptides and functional biological electron-transfer proteins. The present results indicate that the field of metalloprotein design is contributing significantly to the understanding of metals in biology.

引用

收藏

页码：97 / 103

页数：7

相关论文

共 44 条

[1] Emerging principles of de novo catalyst design [J].

Baltzer, L ;

Nilsson, J .

CURRENT OPINION IN BIOTECHNOLOGY, 2001, 12 (04) :355-360

[2] Designing redox metalloproteins from bottom-up and top-down perspectives [J].

Barker, PD .

CURRENT OPINION IN STRUCTURAL BIOLOGY, 2003, 13 (04) :490-499

[3] Experimentally exploring the conformational space sampled by domain reorientation in calmodulin [J].

Bertini, I ;

Del Bianco, C ;

Gelis, I ;

Katsaros, N ;

Luchinat, C ;

Parigi, G ;

Peana, M ;

Provenzani, A ;

Zoroddu, MA .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2004, 101 (18) :6841-6846

[4] Computational design and characterization of a monomeric helical dinuclear metalloprotein [J].

Calhoun, JR ;

Kono, H ;

Lahr, S ;

Wang, W ;

DeGrado, WF ;

Saven, JG .

JOURNAL OF MOLECULAR BIOLOGY, 2003, 334 (05) :1101-1115

[5] De novo design of a cytochrome b maquette for electron transfer and coupled reactions on electrodes [J].

Chen, XX ;

Discher, BM ;

Pilloud, DL ;

Gibney, BR ;

Moser, CC ;

Dutton, PL .

JOURNAL OF PHYSICAL CHEMISTRY B, 2002, 106 (03) :617-624

[6]

COSTANZO LD, 2003, J CHEM SOC DA, P787

[7] Sliding helix and change of coordination geometry in a model Di-MnII protein [J].

DeGrado, WF ;

Di Costanzo, L ;

Geremia, S ;

Lombardi, A ;

Pavone, V ;

Randaccio, L .

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2003, 42 (04) :417-+

[8] Design of a functional protein for molecular recognition:: Specificity of ligand binding in a metal-assembled protein cavity probed by 19F NMR [J].

Doerr, AJ ;

Case, MA ;

Pelczer, I ;

McLendon, GL .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2004, 126 (13) :4192-4198

[9] Hg(II) binding to a weakly associated coiled coil nucleates an encoded metalloprotein fold: A kinetic analysis [J].

Farrer, BT ;

Pecoraro, VL .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (07) :3760-3765

[10] Thermodynamic model for the stabilization of trigonal thiolato mercury(II) in designed three-stranded coiled coils [J].

Farrer, BT ;

Harris, NP ;

Balchus, KE ;

Pecoraro, VL .

BIOCHEMISTRY, 2001, 40 (48) :14696-14705

← 1 2 3 4 5 →