Use of NMR metabolomics to analyze the targets of D-cycloserine in mycobacteria: Role of D-alanine racemase

被引：58

作者：

Halouska, Steven ^{[1
]}

Chacon, Ofelia ^{[2
,3
]}

Fenton, Robert J. ^{[2
]}

Zinniel, Denise K. ^{[2
]}

Barletta, Raul G. ^{[2
]}

Powers, Robert ^{[1
]}

机构：

[1] Univ Nebraska, Dept Chem, Lincoln, NE 68522 USA

[2] Univ Nebraska, Dept Vet & Biomed Sci, Lincoln, NE 68522 USA

[3] Corp Invest Biol, Medellin, Colombia

来源：

JOURNAL OF PROTEOME RESEARCH | 2007年 / 6卷 / 12期

关键词：

NMR; principal component analysis; metabolomics; tuberculosis; D-cycloserine; Mycobacterium smegmatis; D-alanine racemase;

D O I：

10.1021/pr0704332

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

D-Cycloserine (DCS) is only used with multidrug-resistant strains of tuberculosis because of serious side effects. DCS is known to inhibit cell wall biosynthesis, but the in vivo lethal target is still unknown. We have applied NMR-based metabolomics combined with principal component analysis to monitor the in vivo effect of DCS on Mycobacterium smegmatis. Our analysis suggests DCS functions by inhibiting multiple protein targets.

引用

页码：4608 / 4614

页数：7

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[2]

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