Coordinate induction of glutathione biosynthesis and glutathione-metabolizing enzymes is correlated with salt tolerance in tomato

被引：149

作者：

Mittova, V

Theodoulou, FL ^{[1
]}

Kiddle, G

Gómez, L

Volokita, M

Tal, M

Foyer, CH

Guy, M

机构：

[1] Crop Performance & Improvement Dept, Rothamsted Res, Harpenden AL5 2JQ, Herts, England

[2] Ben Gurion Univ Negev, Jacob Blaustein Inst Desert Res, Albert Katz Dept Drylands Biotechnol, IL-84990 Sede Boqer, Israel

[3] Ben Gurion Univ Negev, Dept Life Sci, IL-84105 Beer Sheva, Israel

来源：

FEBS LETTERS | 2003年 / 554卷 / 03期

基金：

英国生物技术与生命科学研究理事会;

关键词：

salt tolerance; tomato; glutathione; 7-glutamylcysteine synthetase; glutathione-S-transferase; glutathione peroxidase;

D O I：

10.1016/S0014-5793(03)01214-6

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The acclimation of reduced glutathione (GSH) biosynthesis and GSH-utilizing enzymes to salt stress was studied in two tomato species that differ in stress tolerance. Salt increased GSH content and GSH:GSSG (oxidized glutathione) ratio in oxidative stress-tolerant Lycopersicon pennellii (Lpa) but not in Lycopersicon esculentum (Lem). These changes were associated with salt-induced upregulation of gamma-glutamylcysteine synthetase protein, an effect which was prevented by preincubation with buthionine sulfoximine. Salt treatment induced glutathione peroxidase and glutathione-S-transferase but not glutathione reductase activities in Lpa. These results suggest a mechanism of coordinate upregulation of synthesis and metabolism of GSH in Lpa, that is absent from Lem. (C) 2003 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：417 / 421

页数：5

共 39 条

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