Root exudates regulate soil fungal community composition and diversty

被引:548
作者
Broeckling, Corey D. [1 ,2 ,3 ]
Broz, Amanda K. [1 ,2 ]
Bergelson, Joy [4 ]
Manter, Daniel K. [1 ,5 ]
Vivanco, Jorge M. [1 ,2 ]
机构
[1] Colorado State Univ, Dept Hort & Landscape Architecture, Ft Collins, CO 80523 USA
[2] Colorado State Univ, Ctr Rhizosphere Biol, Ft Collins, CO 80523 USA
[3] Colorado State Univ, Grad Program Cell & Mol Biol, Ft Collins, CO 80523 USA
[4] Univ Chicago, Dept Ecol & Evolut, Chicago, IL 60637 USA
[5] USDA ARS, Soil Plant Nutrient Res Unit, Ft Collins, CO 80526 USA
关键词
PLANT-MICROBE INTERACTIONS; SINORHIZOBIUM-MELILOTI; MEDICAGO-SATIVA; RHIZOSPHERE; RHIZOBIUM; IDENTIFICATION; SPECIFICITY; EXPRESSION; FLAVONOIDS; SECRETION;
D O I
10.1128/AEM.02188-07
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Plants are in constant contact with a community of soil biota that contains fungi ranging from pathogenic to symbiotic. A few studies have demonstrated a critical role of chemical communication in establishing highly specialized relationships, but the general role for root exudates in structuring the soil fungal community is poorly described. This study demonstrates that two model plant species (Arabidopsis thaliana and Medicago truncatula) are able to maintain resident soil fungal populations but unable to maintain nonresident soil fungal populations. This is mediated largely through root exudates: the effects of adding in vitro-generated root exudates to the soil fungal community were qualitatively and quantitatively similar to the results observed for plants grown in those same soils. This effect is observed for total fungal biomass, phylotype diversity, and overall community similarity to the starting community. Nonresident plants and root exudates influenced the fungal community by both positively and negatively impacting the relative abundance of individual phylotypes. A net increase in fungal biomass was observed when nonresident root exudates were added to resident plant treatments, suggesting that increases in specific carbon substrates and/or signaling compounds support an increased soil fungal population load. This study establishes root exudates as a mechanism through which a plant is able to regulate soil fungal community composition.
引用
收藏
页码:738 / 744
页数:7
相关论文
共 38 条
[1]   Plant sesquiterpenes induce hyphal branching in arbuscular mycorrhizal fungi [J].
Akiyama, K ;
Matsuzaki, K ;
Hayashi, H .
NATURE, 2005, 435 (7043) :824-827
[2]   Two invasive plants alter soil microbial community composition in serpentine grasslands [J].
Batten, KM ;
Scow, KM ;
Davies, KF ;
Harrison, SP .
BIOLOGICAL INVASIONS, 2006, 8 (02) :217-230
[3]   Structural complexity, differential response to infection, and tissue specificity of indolic and phenylpropanoid secondary metabolism in Arabidopsis roots [J].
Bednarek, P ;
Schneider, B ;
Svatos, A ;
Oldham, NJ ;
Hahlbrock, K .
PLANT PHYSIOLOGY, 2005, 138 (02) :1058-1070
[4]   Galactosides in the rhizosphere:: Utilization by Sinorhizobium meliloti and development of a biosensor [J].
Bringhurst, RM ;
Cardon, ZG ;
Gage, DJ .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2001, 98 (08) :4540-4545
[5]   MET-IDEA: Data extraction tool for mass spectrometry-based metabolomics [J].
Broeckling, Corey D. ;
Reddy, Indira R. ;
Duran, Anthony L. ;
Zhao, Xuechun ;
Sumner, Lloyd W. .
ANALYTICAL CHEMISTRY, 2006, 78 (13) :4334-4341
[6]   Plant growth-promoting effects of diazotrophs in the rhizosphere [J].
Dobbelaere, S ;
Vanderleyden, J ;
Okon, Y .
CRITICAL REVIEWS IN PLANT SCIENCES, 2003, 22 (02) :107-149
[7]   Potential role of pathogen signaling in multitrophic plant-microbe interactions involved in disease protection [J].
Duffy, B ;
Keel, C ;
Défago, G .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2004, 70 (03) :1836-1842
[8]   Metabolic profiling and systematic identification of flavonoids and isoflavonoids in roots and cell suspension cultures of Medicago truncatula using HPLC-UV-ESI-MS and GC-MS [J].
Farag, Mohamed A. ;
Huhman, David V. ;
Lei, Zhentian ;
Sumner, Lloyd W. .
PHYTOCHEMISTRY, 2007, 68 (03) :342-354
[9]   ITS PRIMERS WITH ENHANCED SPECIFICITY FOR BASIDIOMYCETES - APPLICATION TO THE IDENTIFICATION OF MYCORRHIZAE AND RUSTS [J].
GARDES, M ;
BRUNS, TD .
MOLECULAR ECOLOGY, 1993, 2 (02) :113-118
[10]  
Hamilton EW, 2001, ECOLOGY, V82, P2397, DOI 10.1890/0012-9658(2001)082[2397:CPSSMA]2.0.CO