Rigidity of the subunit interfaces of the trimeric glutamate transporter GItT during translocation

被引：54

作者：

Groeneveld, Maarten ^{[1
]}

Slotboom, Dirk-Jan ^{[1
]}

机构：

[1] Univ Groningen, Groningen Biomol Sci & Biotechnol Inst, Dept Biochem, NL-9747 AG Groningen, Netherlands

来源：

JOURNAL OF MOLECULAR BIOLOGY | 2007年 / 372卷 / 03期

关键词：

membrane protein; glutamate transport; oligomeric state; transport mechanism; subunit interaction;

D O I：

10.1016/j.jmb.2007.06.067

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Glutamate transporters are trimeric membrane proteins in which each protomer contains a separate translocation path. To determine whether structural rearrangements take place at the subunit interfaces during transport, intersubunit disulfide bridges were introduced in the bacterial transporter GltT. None of the intersubunit cross-links, which had been designed across the entire interface, affected the glutamate transport activity, indicating that the subunit interfaces are rigid during turnover.

引用

收藏

页码：565 / 570

页数：6

相关论文

共 23 条

[1]

[Anonymous], 1971, Methods in Enzymology

[2] Measurement of conformational changes accompanying desensitization in an ionotropic glutamate receptor [J].

Armstrong, Neali ;

Jasti, Jaysankar ;

Beich-Frandsen, Mads ;

Gouaux, Eric .

CELL, 2006, 127 (01) :85-97

[3] Coupling substrate and ion binding to extracellular gate of a sodium-dependent aspartate transporter [J].

Boudker, Olga ;

Ryan, Renae M. ;

Yernool, Dinesh ;

Shimamoto, Keiko ;

Gouaux, Eric .

NATURE, 2007, 445 (7126) :387-393

[4] Purification and reconstitution of the glutamate carrier GltT of the thermophilic bacterium Bacillus stearothermophilus [J].

Gaillard, I ;

Slotboom, DJ ;

Knol, J ;

Lolkema, JS ;

Konings, WN .

BIOCHEMISTRY, 1996, 35 (19) :6150-6156

[5] A trimeric quaternary structure is conserved in bacterial and human glutamate transporters [J].

Gendreau, S ;

Voswinkel, S ;

Torres-Salazar, D ;

Lang, N ;

Heidtmann, H ;

Detro-Dassen, S ;

Schmalzing, G ;

Hidalgo, P ;

Fahlke, C .

JOURNAL OF BIOLOGICAL CHEMISTRY, 2004, 279 (38) :39505-39512

[6] Individual subunits of the glutamate transporter EAAC1 homotrimer function independently of each other [J].

Grewer, C ;

Balani, P ;

Weidenfeller, C ;

Bartusel, T ;

Tao, Z ;

Rauen, T .

BIOCHEMISTRY, 2005, 44 (35) :11913-11923

[7] Intersubunit movement is required for ribosomal translocation [J].

Horan, Lucas H. ;

Noller, Harry F. .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2007, 104 (12) :4881-4885

[8] The glutamate-activated anion conductance in excitatory amino acid transporters is gated independently by the individual subunits [J].

Koch, Hans Peter ;

Brown, Ronald Lane ;

Larsson, Hans Peter .

JOURNAL OF NEUROSCIENCE, 2007, 27 (11) :2943-2947

[9] Small-scale molecular motions accomplish glutamate uptake in human glutamate transporters [J].

Koch, HP ;

Larsson, HP .

JOURNAL OF NEUROSCIENCE, 2005, 25 (07) :1730-1736

[10] The glutamate and chloride permeation pathways are colocalized in individual neuronal glutamate transporter subunits [J].

Leary, Gregory P. ;

Stone, Emily F. ;

Holley, David C. ;

Kavanaugh, Michael P. .

JOURNAL OF NEUROSCIENCE, 2007, 27 (11) :2938-2942