Amperometric immunosensor for the detection of anti-West Nile Virus IgG

被引：51

作者：

Ionescu, Rodica E.

Cosnier, Serge

Herrmann, Sebastien

Marks, Robert S.

机构：

[1] Univ Grenoble 1, CNRS, ICMG FR 2607, Dept Chim Mol UMR 5250, F-38041 Grenoble 9, France

[2] Ben Gurion Univ Negev, Dept Biotechnol Engn, IL-84105 Beer Sheva, Israel

[3] Natl Inst Biotechnol Negev, IL-84105 Beer Sheva, Israel

[4] Ilse Katz Ctr Meso & Nanoscale Sci & Technol, IL-84105 Beer Sheva, Israel

来源：

ANALYTICAL CHEMISTRY | 2007年 / 79卷 / 22期

关键词：

D O I：

10.1021/ac0707129

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An amperometric immunosensor for the detection of West Nile virus (WNV) IgG was developed. This device was based on the immobilization of T7 phages, which were modified by an additional peptide sequence taken from the virus and used as antigen. The electropolymerization of a phage-amphiphilic pyrrole ammonium mixture previously adsorbed on the electrode surface provided an efficient entrapment of phages in a polypyrrole film. After incubation with a secondary peroxidase-labeled antibody, the immunosensors were applied to the quantitative amperometric determination of WW-antibody at 0 V vs Ag/AgCl via the reduction of the enzymically generated quinone in the presence of hydroquinone and H2O2. The optimum immunosensor configuration detected low WNV-antibody dilutions down to a titer of 1:107 with an excellent regeneration of the immunosensor response by glycine treatment.

引用

页码：8662 / 8668

页数：7

共 40 条

[1] ATIAS D, IN PRESS HDB BIOSENS
[2] Prophylactic and therapeutic efficacy of human intravenous immunoglobulin in treating West Nile virus infection in mice
Ben-Nathan, D
Lustig, S
Tam, G
Robinzon, S
Segal, S
Rager-Zisman, B
[J]. JOURNAL OF INFECTIOUS DISEASES, 2003, 188 (01) : 5 - 12
[3] ANTIGENIC RELATIONSHIPS BETWEEN FLAVIVIRUSES AS DETERMINED BY CROSS-NEUTRALIZATION TESTS WITH POLYCLONAL ANTISERA
CALISHER, CH
KARABATSOS, N
DALRYMPLE, JM
SHOPE, RE
PORTERFIELD, JS
WESTAWAY, EG
BRANDT, WE
[J]. JOURNAL OF GENERAL VIROLOGY, 1989, 70 : 37 - 43
[4] IMMOBILIZATION OF REDOX ANIONS IN POLY(AMPHIPHILIC PYRROLYLALKYLAMMONIUM) USING A SIMPLE AND MONOMER-SAVING ONE-STEP PROCEDURE IN PURE WATER-ELECTROLYTE
COCHEGUERENTE, L
DERONZIER, A
GALLAND, B
LABBE, P
MOUTET, JC
REVERDY, G
[J]. JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1991, (06) : 386 - 388
[5] Development of a PPO-poly(amphiphilic pyrrole) electrode for on site monitoring of phenol in aqueous effluents
Cosnier, S
Fombon, JJ
Labbé, P
Limosin, D
[J]. SENSORS AND ACTUATORS B-CHEMICAL, 1999, 59 (2-3) : 134 - 139
[6] Affinity biosensors based on electropolymerized films
Cosnier, S
[J]. ELECTROANALYSIS, 2005, 17 (19) : 1701 - 1715
[7] A NOVEL BIOSENSOR ELABORATION BY ELECTROPOLYMERIZATION OF AN ADSORBED AMPHIPHILIC PYRROLE TYROSINASE ENZYME LAYER
COSNIER, S
INNOCENT, C
[J]. JOURNAL OF ELECTROANALYTICAL CHEMISTRY, 1992, 328 (1-2): : 361 - 366
[8] Electropolymerization of amphiphilic monomers for designing amperometric biosensors
Cosnier, S
[J]. ELECTROANALYSIS, 1997, 9 (12) : 894 - 902
[9] COSNIER S, 1983, BIOELECTROCH BIOENER, V31, P147
[10] COSNIER S, 2006, MATER SCI ENG, V26, P387

← 1 2 3 4 →