Essential role for the interaction between hnRNP H/F and a G quadruplex in maintaining p53 pre-mRNA 3′-end processing and function during DNA damage

被引：141

作者：

Decorsiere, Adrien ^{[1
,2
,3
]}

Cayrel, Anne ^{[1
,2
,3
]}

Vagner, Stephan ^{[1
,2
,3
]}

Millevoi, Stefania ^{[1
,2
,3
]}

机构：

[1] INSERM, U563, F-31000 Toulouse, France

[2] Inst Claudius Regaud, F-31052 Toulouse, France

[3] Univ Toulouse 3, F-31000 Toulouse, France

来源：

GENES & DEVELOPMENT | 2011年 / 25卷 / 03期

关键词：

DNA damage; pre-mRNA 3 '-end processing; polyadenylation; p53 tumor suppressor; hnRNP H; hnRNP F; HETEROGENEOUS NUCLEAR RIBONUCLEOPROTEIN; RIBOSOMAL-PROTEIN L26; MOLECULAR-MECHANISMS; DOWN-REGULATION; POLYMERASE-II; IN-VITRO; TRANSLATION; EXPRESSION; PHOSPHORYLATION; POLYADENYLATION;

D O I：

10.1101/gad.607011

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Following DNA damage, mRNA 39-end formation is inhibited, contributing to repression of mRNA synthesis. Here we investigated how DNA-damaged cells accomplish p53 mRNA 39-end formation when normal mechanisms of pre-mRNA 39-end processing regulation are inhibited. The underlying mechanism involves the interaction between a G-quadruplex structure located downstream from the p53 cleavage site and hnRNP H/F. Importantly, this interaction is critical for p53 expression and contributes to p53-mediated apoptosis. Our results uncover the existence of a specific rescue mechanism of 39-end processing regulation allowing stress-induced p53 accumulation and function in apoptosis.

引用

页码：220 / 225

页数：6

共 34 条

[1] Origins of binding specificity of the A1 heterogeneous nuclear ribonucleoprotein [J].

AbdulMana, N ;

OMalley, SM ;

Williams, KR .

BIOCHEMISTRY, 1996, 35 (11) :3545-3554

[2] The hnRNPs F and H2 bind to similar sequences to influence gene expression [J].

Alkan, SA ;

Martincic, K ;

Milcarek, C .

BIOCHEMICAL JOURNAL, 2006, 393 (01) :361-371

[3] DSEF-1 is a member of the hnRNP H family of RNA-binding proteins and stimulates pre-mRNA cleavage and polyadenylation in vitro [J].

Bagga, PS ;

Arhin, GK ;

Wilusz, J .

NUCLEIC ACIDS RESEARCH, 1998, 26 (23) :5343-5350

[4] Che-1 phosphorylation by ATM/ATR and Chk2 kinases activates p53 transcription and the G2/M checkpoint [J].

Bruno, Tiziana ;

De Nicola, Francesca ;

Iezzi, Simona ;

Lecis, Daniele ;

D'Angelo, Carmen ;

Di Padova, Monica ;

Corbi, Nicoletta ;

Dimiziani, Leopoldo ;

Zannini, Laura ;

Jekimovs, Christian ;

Scarsella, Marco ;

Porrello, Alessandro ;

Chersi, Alberto ;

Crescenzi, Marco ;

Leonetti, Carlo ;

Khanna, Kum Kum ;

Soddu, Silvia ;

Floridi, Aristide ;

Passananti, Claudio ;

Delia, Domenico ;

Fanciulli, Maurizio .

CANCER CELL, 2006, 10 (06) :473-486

[5] CPEB regulation of human cellular senescence, energy metabolism, and p53 mRNA translation [J].

Burns, David M. ;

Richter, Joel D. .

GENES & DEVELOPMENT, 2008, 22 (24) :3449-3460

[6] P68 RNA Helicase (DDX5) Alters Activity of Cis- and Trans-Acting Factors of the Alternative Splicing of H-Ras [J].

Camats, Maria ;

Guil, Sonia ;

Kokolo, Mariette ;

Bach-Elias, Montse .

PLOS ONE, 2008, 3 (08)

[7] Nuclear deadenylation/polyadenylation factors regulate 3′ processing in response to DNA damage [J].

Cevher, Murat A. ;

Zhang, Xiaokan ;

Fernandez, Sully ;

Kim, Sergey ;

Baquero, Jorge ;

Nilsson, Per ;

Lee, Sean ;

Virtanen, Anders ;

Kleiman, Frida E. .

EMBO JOURNAL, 2010, 29 (10) :1674-1687

[8] Auxiliary downstream elements are required for efficient polyadenylation of mammalian pre-mRNAs [J].

Chen, F ;

Wilusz, J .

NUCLEIC ACIDS RESEARCH, 1998, 26 (12) :2891-2898

[9] Two G-rich regulatory elements located adjacent to and 440 nucleotides downstream of the core poly(A) site of the intronless melanocortin receptor 1 gene are critical for efficient 3′ end processing [J].

Dalziel, Martin ;

Nunes, Nuno Miguel ;

Furger, Andre .

MOLECULAR AND CELLULAR BIOLOGY, 2007, 27 (05) :1568-1580

[10] 3′ end mRNA processing:: molecular mechanisms and implications for health and disease [J].

Danckwardt, Sven ;

Hentze, Matthias W. ;

Kulozik, Andreas E. .

EMBO JOURNAL, 2008, 27 (03) :482-498

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