Expression of ligands to NKp46 in benign and malignant melanocytes

被引:35
作者
Cagnano, Emanuela [2 ]
Hershkovitz, Oren [1 ,3 ]
Zilka, Alon [1 ,3 ]
Bar-Ilan, Ahuva [1 ,3 ]
Golder, Alexandra [1 ,3 ]
Sion-Vardy, Netta [2 ]
Bogdanov-Berezovsky, Alexander [4 ]
Mandelboim, Ofer [5 ]
Benharroch, Daniel [2 ]
Porgador, Angel [1 ,3 ]
机构
[1] Ben Gurion Univ Negev, Fac Hlth Sci, Dept Microbiol & Immunol, IL-84105 Beer Sheva, Israel
[2] Soroka Univ Med Ctr, Dept Pathol, Beer Sheva, Israel
[3] Ben Gurion Univ Negev, Canc Res Ctr, IL-84105 Beer Sheva, Israel
[4] Soroka Univ Med Ctr, Dept Plast Surg, Beer Sheva, Israel
[5] Hebrew Univ Jerusalem, Hadassah Med Sch, Lautenberg Ctr Gen & Tumor Immunol, IL-91010 Jerusalem, Israel
关键词
D O I
10.1038/sj.jid.5701111
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
Human melanoma cell lines were shown to express ligands for the natural cytotoxicity receptor, NKp46, expressed by natural killer (NK) cells. We aimed to examine the expression of ligands for NKp46 by various primary human melanocytic cells and melanocytic lesions. Sections from primary nevi and melanomas were tested for expression of NKp46 ligands employing chimeric NKp46-Fc for staining. The melanocytes present in the reticular dermis were negative for NKp46 ligands in common nevi; in malignant melanocytic lesions, the deeper melanocytes were focally positive. In dermoepidermal junction of all melanocytic lesions, the melanocytes showed enhanced expression of NKp46 ligands. Melanophages in all lesions were consistently positive for NKp46 ligands. These observations establish the expression of NKp46 ligands by primary-transformed melanocytes. Normal melanocytes did not express ligands to NKp46. Therefore, the results show (i) a correlation between the malignant potential of the lesion and the expression of NKp46 ligands in the reticular dermis, and (ii) enhanced expression of NKp46 ligands in the active proliferation zone (dermoepidermal junction) of nevi and melanomas. Ligands to NKp46 were expressed on the membrane and within the cells. The physiological role of NKp46 ligands in the progression of malignancy within melanocytic lesions should be explored further.
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收藏
页码:972 / 979
页数:8
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