Establishment of poly(ADP-ribose) polymerase-deficient mouse embryonic stem cell lines

被引：12

作者：

Masutani, M ^{[1
]}

Nozaki, T

Nishiyama, E

Ochiya, T

Nakagama, H

Wakabayashi, K

Suzuki, H

Sugimura, T

机构：

[1] Natl Canc Ctr, Res Inst, Div Biochem, Chuo Ku, Tokyo 1040045, Japan

[2] Natl Canc Ctr, Res Inst, Sect Studies Metastasis, Chuo Ku, Tokyo 1040045, Japan

[3] Natl Canc Ctr, Res Inst, Can Prevent Div, Chuo Ku, Tokyo 1040045, Japan

[4] Chugai Pharmaceut Co Ltd, Shizuoka 4120038, Japan

来源：

PROCEEDINGS OF THE JAPAN ACADEMY SERIES B-PHYSICAL AND BIOLOGICAL SCIENCES | 1998年 / 74卷 / 10期

关键词：

Gene-targeting; poly(ADP-ribose) polymerase; embryonic stem cells;

D O I：

10.2183/pjab.74.233

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Many studies revealed that poly(ADP-ribose) polymerase (Parp) is involved in DNA-damage recovery, cell-death induction and maintenance of genomic stability. We generated mouse Parp(+/-) embryonic stem (ES) cell lines by disrupting one allele of Parp exon 1 with a neomycin-resistance gene-cassette and subsequently produced Parp(-/-) ES cells by disrupting the remaining allele with a puromycin-resistance gene-cassette. Parp activity was decreased to half in Parp(+/-) ES cell clones and lost in Parp(-/-) ES cell clones. Growth rates of Parp(+/-) and Parp(-/-) ES cell clones were similar to that of parental J1 ES cells, indicating that Parp is not required for ES cell proliferation. These Parp(-/-) ES cells will be useful to study biological role of poly(ADP-ribosyl)ation reaction.

引用

页码：233 / 236

页数：4

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